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Titolo:
A DNA-BINDING PROTEIN FROM CANDIDA-ALBICANS THAT BINDS TO THE RPG BOXOF SACCHAROMYCES-CEREVISIAE AND THE TELOMERIC REPEAT SEQUENCE OF CANDIDA-ALBICANS
Autore:
ISHII N; YAMAMOTO M; LAHM HW; IIZUMI S; YOSHIHARA F; NAKAYAMA H; ARISAWA M; AOKI Y;
Indirizzi:
NIPPON ROCHE RES CTR,DEPT MYCOL,KAJIWARA 200 KAMAKURA KANAGAWA 247 JAPAN NIPPON ROCHE RES CTR,DEPT MYCOL KAMAKURA KANAGAWA 247 JAPAN HOFFMANN LA ROCHE AG,DEPT PRPB CH-4002 BASEL SWITZERLAND
Titolo Testata:
Microbiology
, volume: 143, anno: 1997,
parte:, 2
pagine: 417 - 427
SICI:
1350-0872(1997)143:<417:ADPFCT>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
FUNCTIONAL-ANALYSIS; TRANSCRIPTIONAL ACTIVATORS; SUCROSE UTILIZATION; YEAST; CLONING; GENE; EXPRESSION; SYSTEM; RAP1; PURIFICATION;
Keywords:
CANDIDA ALBICANS; TRANSCRIPTION FACTOR; RPG BOX; NUCLEAR PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
N. Ishii et al., "A DNA-BINDING PROTEIN FROM CANDIDA-ALBICANS THAT BINDS TO THE RPG BOXOF SACCHAROMYCES-CEREVISIAE AND THE TELOMERIC REPEAT SEQUENCE OF CANDIDA-ALBICANS", Microbiology, 143, 1997, pp. 417-427

Abstract

Electromobility shift assays with a DNA probe containing the Saccharomyces cerevisiae ENO1 RPG box identified a specific DNA-binding protein in total protein extracts of Candida albicans. The protein, named Rbf1p (RPG-box-binding protein 1), bound to other S. cerevisiae RPG boxes, although the nucleotide recognition profile was not completely the same as that of S. cerevisiae Rap1p (repressor-activator protein 1), an RPG-box-binding protein. The repetitive sequence of the C. albicans chromosomal telomere also competed with RPG-box binding to Rbf1p. For further analysis, we purified Rbf1p 57 600-fold from C. albicans totalprotein extracts, raised mAbs against the purified protein and immunologically cloned the gene, whose ORF specified a protein of 527 aa. The bacterially expressed protein showed RPG-box-binding activity with the same profile as that of the purified one. The Rbf1p, containing twoglutamine-rich regions that are found in many transcription factors, showed transcriptional activation capability in S. cerevisiae and was predominantly observed in nuclei. These results suggest that Rbf1p is a transcription factor with telomere-binding activity in C. albicans.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/01/21 alle ore 18:35:20