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Titolo:
NUCLEOTIDE-SEQUENCE COMPARISON OF THE MYCOBACTERIAL DNAJ GENE AND PCR-RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS FOR IDENTIFICATION OF MYCOBACTERIAL SPECIES
Autore:
TAKEWAKI S; OKUZUMI K; MANABE I; TANIMURA M; MIYAMURA K; NAKAHARA K; YAZAKI Y; OHKUBO A; NAGAI R;
Indirizzi:
UNIV TOKYO,DEPT INTERNAL MED 3,7-3-1 HONGO,BUNKYO KU TOKYO 113 JAPAN UNIV TOKYO,DEPT INTERNAL MED 3,7-3-1 HONGO,BUNKYO KU TOKYO 113 JAPAN UNIV TOKYO,DEPT LAB MED,BUNKYO KU TOKYO 113 JAPAN NATL CHILDRENS MED RES CTR,DEPT CHILD ECOL,SETAGAYA KU TOKYO 154 JAPAN NATL INST HLTH,DEPT EPIDEMIOL,SHINJUKU TOKYO 162 JAPAN
Titolo Testata:
International journal of systematic bacteriology
fascicolo: 1, volume: 44, anno: 1994,
pagine: 159 - 166
SICI:
0020-7713(1994)44:1<159:NCOTMD>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; DEOXYRIBONUCLEIC-ACID RELATEDNESS; AVIUM COMPLEX; GROWING MYCOBACTERIA; CLINICAL-SAMPLES; RAPID DIAGNOSIS; TUBERCULOSIS; AMPLIFICATION; HYBRIDIZATION; PROTEINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
S. Takewaki et al., "NUCLEOTIDE-SEQUENCE COMPARISON OF THE MYCOBACTERIAL DNAJ GENE AND PCR-RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS FOR IDENTIFICATION OF MYCOBACTERIAL SPECIES", International journal of systematic bacteriology, 44(1), 1994, pp. 159-166

Abstract

We recently reported a genus-specific PCR for the mycobacterial dnaJ gene. In the present study, we have determined the nucleotide sequences of the dnaJ gene from 19 mycobacterial species (Mycobacterium tuberculosis, M. bovis, M. bovis BCG, M. africanum, M. microti, M. marinum, M. kansasii, M. gastri, M. simiae, M. scrofulaceum, M. szulgai, M. gordonae, M. avium, M. intracellulare, M. xenopi, M. fortuitum, M. chelonae, M. hemophilum, and M. paratuberculosis). On the basis of the amplified dnaJ gene nucleotide sequences, we constructed a phylogenetic tree of the mycobacterial species by using the neighbor-joining method and unweighted pairwise grouping method of arithmetic average. We found that the phylogenetic relationship inferred within the slowly growing species was in good agreement with the traditional classification, with three major branches corresponding to Runyon's groups I, II, and III. An exception was M. simiae, which was phylogenetically closer to thecluster including members of Runyon's group III than to that of Runyon's group I. On the other band, the rapid growers, such as M. fortuitum and M. chelonae, did not form a coherent line corresponding to Runyon's group IV, indicating that our phylogenetic analysis based on the dnaj gene reflects the phenotypic characteristics such as pigmentation but not the growth rate. Finally, we revealed the species-specific restriction sites within the amplified dnaJ gene to differentiate most ofthe mycobacterial DNA by a combination of PCR with restriction fragment length polymorphism analysis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 05:42:16