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Titolo:
IDENTIFICATION AND DETECTION OF TRYPANOSOMA-CRUZI BY USING A DNA AMPLIFICATION FINGERPRINT OBTAINED FROM THE RIBOSOMAL INTERGENIC SPACER
Autore:
GONZALEZ N; GALINDO I; GUEVARA P; NOVAK E; SCORZA JV; ANEZ N; DASILVEIRA JF; RAMIREZ JL;
Indirizzi:
UNIV CENT VENEZUELA,FAC CIENCIAS,GENET MOLEC GRP,APDO 47525 CARACAS 1041A VENEZUELA UNIV CENT VENEZUELA,FAC CIENCIAS,GENET MOLEC GRP,APDO 47525 CARACAS 1041A VENEZUELA GERENCIA NACL BIOTECNOL CARACAS VENEZUELA FAC CIENCIAS MERIDA MERIDA VENEZUELA NUCL UNIV RAFAEL RANGEL TRUJILLO VENEZUELA UNIV LOS ANDES MERIDA VENEZUELA ESCOLA PAULISTA MED,DEPT IMMUNOL & PARASITOL BR-04023 SAO PAULO BRAZIL
Titolo Testata:
Journal of clinical microbiology
fascicolo: 1, volume: 32, anno: 1994,
pagine: 153 - 158
SICI:
0095-1137(1994)32:1<153:IADOTB>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; CHAGAS-DISEASE; GENE SPACER; LEISHMANIA; DIAGNOSIS; CLASSIFICATION; SEQUENCES; STRAIN; ASSAY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
N. Gonzalez et al., "IDENTIFICATION AND DETECTION OF TRYPANOSOMA-CRUZI BY USING A DNA AMPLIFICATION FINGERPRINT OBTAINED FROM THE RIBOSOMAL INTERGENIC SPACER", Journal of clinical microbiology, 32(1), 1994, pp. 153-158

Abstract

We designed a PCR assay targeted on repeated elements of the ribosomal intergenic spacer which produces highly polymorphic DNA band patterns for different strains of Trypanosoma cruzi. By labeling the PCR products with digoxigenin and by chemiluminescence detection, we improved the assay sensitivity by three orders of magnitude to get T. cruzi strain fingerprints in feces of the trypanosome-infected triatomine bug vector. We also developed a capture assay for the digoxigenin-labeled PCR products that allowed us to detect T. cruzi in triatomine bug vector feces and in human serum samples with a solid support.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 16:53:47