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Titolo:
DEMONSTRATION OF BORNA-DISEASE VIRUS-SPECIFIC RNA IN SECRETIONS OF NATURALLY INFECTED HORSES BY THE POLYMERASE CHAIN-REACTION
Autore:
RICHT JA; HERZOG S; HABERZETTL K; ROTT R;
Indirizzi:
UNIV GIESSEN,INST VIROL,FRANKFURTER STR 107 D-35392 GIESSEN GERMANY
Titolo Testata:
Medical microbiology and immunology
fascicolo: 6, volume: 182, anno: 1993,
pagine: 293 - 304
SICI:
0300-8584(1993)182:6<293:DOBVRI>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
AGENT; PROTEINS; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
J.A. Richt et al., "DEMONSTRATION OF BORNA-DISEASE VIRUS-SPECIFIC RNA IN SECRETIONS OF NATURALLY INFECTED HORSES BY THE POLYMERASE CHAIN-REACTION", Medical microbiology and immunology, 182(6), 1993, pp. 293-304

Abstract

The presence of Borna disease virus (BDV)-specific RNA was traced by the reverse transcriptase-polymerase chain reaction in conjunctival fluid, nasal secretions and saliva of horses which were seropositive butdid not have any history of clinical Borna disease. Positive reactions encompassed sequences encoding the p24 BDV-specific protein. Virus specificity of the amplified product was confirmed by hybridization with the respective oligomer probe. Viral infectivity or virus-specific antigen was not found in any of these secretions by conventional assaysin cell culture and immunoblotting.

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Documento generato il 21/01/20 alle ore 00:54:14