Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
MULTIPLE FORMS OF GELATINASES TYPE-IV COLLAGENASES IN SALIVA AND GINGIVAL CREVICULAR FLUID OF PERIODONTITIS PATIENTS
Autore:
INGMAN T; SORSA T; LINDY O; KOSKI H; KONTTINEN YT;
Indirizzi:
UNIV HELSINKI,DEPT ANAT,SILTAVUORENPENGRP 20A SF-00170 HELSINKI 17 FINLAND UNIV HELSINKI,DEPT MED CHEM SF-00170 HELSINKI 17 FINLAND UNIV HELSINKI,DEPT PERIODONTOL SF-00170 HELSINKI 17 FINLAND
Titolo Testata:
Journal of clinical periodontology
fascicolo: 1, volume: 21, anno: 1994,
pagine: 26 - 31
SICI:
0303-6979(1994)21:1<26:MFOGTC>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMORPHONUCLEAR LEUKOCYTE COLLAGENASE; TISSUE INHIBITOR; INTERSTITIAL COLLAGENASES; MATRIX METALLOPROTEINASES; TETRACYCLINE-INHIBITION; JUVENILE PERIODONTITIS; LATENT COLLAGENASE; HUMAN-SKIN; PURIFICATION; ORIGIN;
Keywords:
ELASTASE; GELATINASE; GINGIVAL CREVICULAR FLUID; PERIODONTITIS; SALIVA; ZYMOGRAPHY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
T. Ingman et al., "MULTIPLE FORMS OF GELATINASES TYPE-IV COLLAGENASES IN SALIVA AND GINGIVAL CREVICULAR FLUID OF PERIODONTITIS PATIENTS", Journal of clinical periodontology, 21(1), 1994, pp. 26-31

Abstract

The aim of the present study was to characterize the eventual presence and molecular forms of gelatinase/type IV collagenase activities in gingival crevicular fluid (GCF) and saliva in different forms of periodontitis; patients with clinically healthy periodontium served as controls. Enzyme activities were monitored electrophoretically by zymography using gelatin and type IV collagen as substrates and analyzed visually and/or densitometrically. Both saliva and GCF collected from adultperiodontitis, localized juvenile periodontitis and type II diabetes mellitus periodontitis patients contained species moving identically with gelatinase isolated from human neutrophils or MMP-9 (mean 98 kD), and species with mobility similar to gelatinase in fibroblast cell culture supernatants or MMP-2 (mean 76 kD). Hitherto, undescribed high molecular weight forms (mean 128 kD), were found, possibly representing polymerized or complexed enzyme active/activated in situ in the gel matrix. Small molecular forms of gelatinases (mean 51 kD and 46 kD), unable to cleave type IV collagen, were also found, most likely representing in vivo proteolytically activated, truncated enzymes. Although multiple forms of gelatinases/type IV collagenases in saliva and GCF may take part in the tissue destruction in periodontitis, their profile judged according to molecular weights does not differentiate between different forms of periodontitis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/08/20 alle ore 08:28:23