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Titolo:
EVIDENCE FOR A ROLE OF THE DROSOPHILA-MELANOGASTER-SUPPRESSOR-OF-SABLE GENE IN THE PREMESSENGER RNA SPLICING PATHWAY
Autore:
FRIDELL RA; SEARLES LL;
Indirizzi:
UNIV N CAROLINA,DEPT BIOL CHAPEL HILL NC 27599 UNIV N CAROLINA,DEPT BIOL CHAPEL HILL NC 27599
Titolo Testata:
Molecular and cellular biology
fascicolo: 1, volume: 14, anno: 1994,
pagine: 859 - 867
SICI:
0270-7306(1994)14:1<859:EFAROT>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
VERMILION GENE; BINDING PROTEINS; WHITE LOCUS; DNA; SEQUENCES; PROMOTER; ALLELES; HYBRIDIZATION; TRANSCRIPTION; REGULATORS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
R.A. Fridell e L.L. Searles, "EVIDENCE FOR A ROLE OF THE DROSOPHILA-MELANOGASTER-SUPPRESSOR-OF-SABLE GENE IN THE PREMESSENGER RNA SPLICING PATHWAY", Molecular and cellular biology, 14(1), 1994, pp. 859-867

Abstract

Recessive mutations of the Drosophila melanogaster suppressor of sable [su(s)] gene result in elevated accumulation of RNA from vermilion (v) mutant alleles that have an insertion of the 7.5-kb retrotransposon412 in the first exon of the v gene. During transcription of such a vmutant gene, the 412 sequences are incorporated into the primary transcripts and are subsequently removed by splicing at cryptic sites within 412 sequences. In a su(s)+ background, the level of these unusuallyspliced transcripts is exceedingly low, and su(s) mutations increase their accumulation. We previously proposed that v R-NA levels are elevated in su(s) mutants because of increased recognition of the cryptic splice sites, and the aim of this study was to test this hypothesis. We generated a v mutant derivative with a smaller 412 insertion, introduced alterations into the 412-associated splice sites, and examined the effect of su(s) mutations on expression of these derivatives after germ line transformation. To increase overall expression levels, the v promoter was replaced with the stronger Metallothionein (Mtn) gene promoter. We found that transformants bearing a v derivative with 480 bp of 412 sequences accumulate both transcripts, with 412 sequences spliced out and transcripts that retain 412 sequences. Mutations of su(s) increase the levels of both transcript classes without affecting the relative amounts of the two forms. Strikingly, replacement of the cryptic 5' splice sites with a 5' consensus produces the same effect as, andeliminates the response to, a su(s) mutation. In addition, we demonstrated that mutations of su(s) lead to increased accumulation of v transcripts even when the previously identified cryptic 412 5' and 3' splice sites were destroyed and that other cryptic splice sites reside within Mtn and 412 sequences. These results indicate that the v mutant transcripts are stabilized by assembly of the 412 sequences into splicing complexes and support the hypothesis that splicing complexes more readily assemble on cryptic splice sites in su(s) mutants.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 04:25:52