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Titolo:
HEAT-SHOCK FACTOR CAN ACTIVATE TRANSCRIPTION WHILE BOUND TO NUCLEOSOMAL DNA IN SACCHAROMYCES-CEREVISIAE
Autore:
PEDERSON DS; FIDRYCH T;
Indirizzi:
UNIV VERMONT,SCH MED,DEPT MICROBIOL & MOLEC GENET,STAFFORD BLDG BURLINGTON VT 05405 UNIV VERMONT,SCH MED,MARKEY CTR MOLEC GENET BURLINGTON VT 05405
Titolo Testata:
Molecular and cellular biology
fascicolo: 1, volume: 14, anno: 1994,
pagine: 189 - 199
SICI:
0270-7306(1994)14:1<189:HFCATW>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR VIRUS PROMOTER; CORE PARTICLE; HYPERSENSITIVE SITES; CHROMATIN STRUCTURE; TATA ELEMENTS; YEAST; GENE; BINDING; INVITRO; INVIVO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
63
Recensione:
Indirizzi per estratti:
Citazione:
D.S. Pederson e T. Fidrych, "HEAT-SHOCK FACTOR CAN ACTIVATE TRANSCRIPTION WHILE BOUND TO NUCLEOSOMAL DNA IN SACCHAROMYCES-CEREVISIAE", Molecular and cellular biology, 14(1), 1994, pp. 189-199

Abstract

After each round of replication, new transcription initiation complexes must assemble on promoter DNA. This process may compete with packaging of the same promoter sequences into nucleosomes. To elucidate interactions between regulatory transcription factors and nucleosomes on newly replicated DNA, we asked whether heat shock factor (HSF) could bemade to bind to nucleosomal DNA in vivo. A heat shock element (HSE) was embedded at either of two different sites within a DNA segment thatdirects the formation of a stable, positioned nucleosome. The resulting DNA segments were coupled to a reporter gene and transfected into the yeast Saccharomyces cerevisiae. Transcription from these two plasmid constructions after induction by heat shock was similar in amount tothat from a control plasmid in which HSF binds to nucleosome-free DNA. High-resolution genomic footprint mapping of DNase I and micrococcalnuclease cleavage sites indicated that the HSE in these two plasmids was, nevertheless, packaged in a nucleosome. The inclusion of HSE sequences within (but relatively close to the edge of) the nucleosome did not alter the position of the nucleosome which formed with the parental DNA fragment. Genomic footprint analyses also suggested that the HSE-containing nucleosome was unchanged by the induction of transcription. Quantitative comparisons with control plasmids ruled out the possibility that HSF was bound only to a small fraction of molecules that might have escaped nucleosome assembly. Analysis of the helical orientation of HSE DNA in the nucleosome indicated that HSF contacted DNA residues that faced outward from the histone octamer. We discuss the significance of these results with regard to the role of nucleosomes in inhibiting transcription and the normal occurrence of nucleosome-free regions in promoters.

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Documento generato il 02/04/20 alle ore 21:54:18