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Titolo:
CHARACTERIZATION OF A SINGLE STRONG TISSUE-SPECIFIC ENHANCER DOWNSTREAM FROM THE 3 HUMAN GENES ENCODING PLACENTAL-LACTOGEN
Autore:
JACQUEMIN P; OURY C; PEERS B; MORIN A; BELAYEW A; MARTIAL JA;
Indirizzi:
UNIV LIEGE,INST CHIM,BIOL MOLEC & GENIE GENET LAB,B-6 B-4000 SART BELGIUM UNIV LIEGE,INST CHIM,BIOL MOLEC & GENIE GENET LAB,B-6 B-4000 SART BELGIUM
Titolo Testata:
Molecular and cellular biology
fascicolo: 1, volume: 14, anno: 1994,
pagine: 93 - 103
SICI:
0270-7306(1994)14:1<93:COASST>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-GROWTH-HORMONE; TRANS-ACTING FACTOR; TRANSCRIPTIONAL ENHANCER; SV40 ENHANCER; CHORIONIC SOMATOMAMMOTROPIN; NUCLEOTIDE-SEQUENCE; RESPONSE ELEMENT; BINDING-PROTEINS; THYROID-HORMONE; MAMMALIAN-CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
P. Jacquemin et al., "CHARACTERIZATION OF A SINGLE STRONG TISSUE-SPECIFIC ENHANCER DOWNSTREAM FROM THE 3 HUMAN GENES ENCODING PLACENTAL-LACTOGEN", Molecular and cellular biology, 14(1), 1994, pp. 93-103

Abstract

The human genes coding for growth hormone (hGH) and placental lactogen (choriosomatomammotropic hormone [hCS]) are clustered on chromosome 17 in the following order: 5' hGH-N hCS-L hCS-A hGH-V hCS-B 3'. So far, a single placenta-specific enhancer has been identified in the locus, 2 kb downstream from the hCS-B gene, and shown to comprise one in vitro binding site for a nuclear protein. We here provide evidence that the hCS-B enhancer is more complex: (i) protection against DNase I digestion in the 3' flanking region of the hCS-B gene reveals four binding sites (DF-1, DF-2, DF-3, and DF-4) for nuclear proteins from either placental or HeLa cells, and (ii) placenta-specific enhancer activity can be fully exerted in transient expression experiments by a 126-bp fragment comprising the DF-3 and DF-4 protein-binding sites. By dissecting this region, we show that enhancer activity is mediated by a synergy between DF-3 and DF-4. Competitions with various oligonucleotides in footprinting and gel retardation experiments indicate that the same protein or set of proteins, different in HeLa and placenta cell nuclei, interacts with sites DF-2, DF-3, and DF-4. We also studied the regions of the hCS-L and hCS-A genes which are highly similar to the hCS-B enhancer. Although they each present the same four protein-binding sites, they exhibit only minor enhancer activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 16:39:30