Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
MAINTENANCE OF VIABILITY AND TRANSPORT FUNCTION AFTER PRESERVATION OFISOLATED RAT HEPATOCYTES IN VARIOUS SIMPLIFIED UNIVERSITY-OF-WISCONSIN SOLUTIONS
Autore:
SANDKER GW; WEERT B; MEREMA MT; KUIPERS W; SLOOFF MJH; MEIJER DKF; GROOTHUIS GMM;
Indirizzi:
UNIV GRONINGEN,CTR PHARM,DEPT PHARMACOL & THERAPEUT,ANTONIUS DEUSINGLAAN 2 9713 AW GRONINGEN NETHERLANDS UNIV GRONINGEN,CTR PHARM,DEPT PHARMACOL & THERAPEUT 9713 AW GRONINGENNETHERLANDS UNIV HOSP GRONINGEN,DEPT SURG GRONINGEN NETHERLANDS
Titolo Testata:
Biochemical pharmacology
fascicolo: 11, volume: 46, anno: 1993,
pagine: 2093 - 2096
SICI:
0006-2952(1993)46:11<2093:MOVATF>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
SIMPLE COLD-STORAGE; UW-SOLUTION; LIVER PRESERVATION; KIDNEY-PRESERVATION; HYDROXYETHYL STARCH; HYPOTHERMIC PRESERVATION; METABOLIC FUNCTION; CELL VIABILITY; CANINE LIVER; EURO-COLLINS;
Tipo documento:
Note
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
G.W. Sandker et al., "MAINTENANCE OF VIABILITY AND TRANSPORT FUNCTION AFTER PRESERVATION OFISOLATED RAT HEPATOCYTES IN VARIOUS SIMPLIFIED UNIVERSITY-OF-WISCONSIN SOLUTIONS", Biochemical pharmacology, 46(11), 1993, pp. 2093-2096

Abstract

Rat hepatocytes were preserved for 24hr with high recovery and good maintenance of viability and transport function both in University of Wisconsin (UW) solution and in various simplified UW solutions. Cell quality is somewhat affected after 48 hr of preservation in both the original UW solution and the simplified solutions. ATP content and uptakerate of taurocholic acid are more sensitive markers of cell viabilitythan Trypan blue exclusion or the MTT test. A much less expensive solution than UW, containing only K+-lactobionate, KH2PO4, MgSO4 and raffinose, can be used successfully for preservation of rat hepatocytes for 24 hr for drug transport studies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 08:09:16