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Titolo:
THE PROTON PERMEABILITY OF LIPOSOMES MADE FROM MITOCHONDRIAL INNER MEMBRANE PHOSPHOLIPIDS - COMPARISON WITH ISOLATED-MITOCHONDRIA
Autore:
BROOKES PS; ROLFE DFS; BRAND MD;
Indirizzi:
UNIV CAMBRIDGE,DEPT BIOCHEM,TENNIS COURT RD CAMBRIDGE CB2 1QW ENGLAND
Titolo Testata:
The Journal of membrane biology
fascicolo: 2, volume: 155, anno: 1997,
pagine: 167 - 174
SICI:
0022-2631(1997)155:2<167:TPPOLM>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
LARGE UNILAMELLAR VESICLES; ELECTRON-TRANSPORT; LIVER-MITOCHONDRIA; LIPID BILAYER; RAT-LIVER; CONDUCTANCE; LEAK; DISCRIMINATION; METABOLISM; MECHANISMS;
Keywords:
MITOCHONDRIA; VESICLE; PROTON LEAK; PHOSPHOLIPID; LIPOSOME; FATTY ACID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
P.S. Brookes et al., "THE PROTON PERMEABILITY OF LIPOSOMES MADE FROM MITOCHONDRIAL INNER MEMBRANE PHOSPHOLIPIDS - COMPARISON WITH ISOLATED-MITOCHONDRIA", The Journal of membrane biology, 155(2), 1997, pp. 167-174

Abstract

Unilamellar liposomes with native phospholipid fatty acid compositionwere prepared from rat liver mitochondrial inner membrane phospholipids by extrusion in medium containing 50 mM potassium. They were diluted into low potassium medium to establish a transmembrane potassium gradient. A known membrane potential was imposed by addition of valinomycin, and proton flux into liposomes was measured. Valinomycin in the range 10 pM-1nM was sufficient to fully establish membrane potential. Valinomycin concentrations above 3 nn? catalyzed additional proton flux and were avoided. At 300 pM valinomycin, proton flux depended nonlinearly on membrane potential. At 160 mV membrane potential the flux was 30 nmol H+/min/mg phospholipid-approximately 5% of the proton leak fluxunder comparable conditions in isolated mitochondria, indicating thatleak pathways through bulk phospholipid bilayer account for only a small proportion of total mitochondrial proton leak.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 11:54:31