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Titolo:
A PRELIMINARY TRIAL OF HUMAN ZYGOTE CULTURE IN HAM F-10 WITHOUT HYPOXANTHINE
Autore:
LOUTRADIS DC; KIESSLING AA; KALLIANIDIS K; SISKOS K; CREATSAS G; MICHALAS S; ARAVANTINOS D;
Indirizzi:
HARVARD UNIV,SCH MED,FAULKNER CTR REPROD MED,1153 CTR ST BOSTON MA 02115 UNIV ATHENS,ALEXANDRA MATERN HOSP,SCH MED,DEPT OBSTET & GYNECOL ATHENS GREECE
Titolo Testata:
Journal of assisted reproduction and genetics
fascicolo: 4, volume: 10, anno: 1993,
pagine: 271 - 275
SICI:
1058-0468(1993)10:4<271:APTOHZ>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Keywords:
IN-VITRO FERTILIZATION (IVF); CULTURE MEDIUM; HYPOXANTHINE; EARLY EMBRYO DEVELOPMENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
NO
Recensione:
Indirizzi per estratti:
Citazione:
D.C. Loutradis et al., "A PRELIMINARY TRIAL OF HUMAN ZYGOTE CULTURE IN HAM F-10 WITHOUT HYPOXANTHINE", Journal of assisted reproduction and genetics, 10(4), 1993, pp. 271-275

Abstract

Purpose: This study deals with the suitability of Ham's F-10 without hypoxanthine for early cleavage-stage embryo culture. A high percentage of mouse two-cell embryos developed into morula and blastocysts in Ham's F-10 formulated without hypoxanthine (75.3 and 71.6%, respectively); in contrast, in agreement with previous reports, only 15.4% developed beyond the two-cell stage in Ham's F-10 with hypoxanthine. To begin to evaluate the effect of hypoxanthine on human embryos, a total of 318 human oocytes was fertilized and cultured in Ham's F-10 minus hypoxanthine. Results: The fertilization, cleavage, and pregnancy percentages in two ovulation induction protocols [human menopausal gonadotropin (hMG)/human chorionic gonadotropin (hCG) and gonadotropin releasing hormone/hMG/hCG] were 82, 88, and 20 and 83, 91, and 30%, respectively. Conclusion: These results suggest that, in agreement with mouse embryo development, hypoxanthine does not appear to be necessary for humanembryo cleavage and its omission from Ham's F-10 may enhance the conditions for culture of early-cleaving human embryos. These observationsmay lead to a better understanding of critical cell processes during early human embryonic development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 21:28:37