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Titolo:
ARE LONG-TERM CRYOPRESERVATION AND PATENCY OF VEIN ALLOGRAFT TRULY ACHIEVABLE
Autore:
PASQUINELLI G; FAGGIOLI GL; PREDA P; VICI M; MARTINELLI GN; GIARDINO R; DADDATO M; RICOTTA JJ;
Indirizzi:
OSPED S ORSOLA MALPIGHI,INST CLIN ELECTRON MICROSCOPY,VIA MASSARENTI 9 I-40138 BOLOGNA ITALY UNIV BOLOGNA,INST CLIN ELECTRON MICROSCOPY I-40126 BOLOGNA ITALY UNIV BOLOGNA,INST EXPTL SURG I-40126 BOLOGNA ITALY UNIV BOLOGNA,DEPT VASC SURG I-40126 BOLOGNA ITALY SUNY BUFFALO,DEPT SURG,DIV VASC SURG BUFFALO NY 14260
Titolo Testata:
Cells and materials
fascicolo: 3, volume: 3, anno: 1993,
pagine: 305 - 314
SICI:
1051-6794(1993)3:3<305:ALCAPO>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
VENOUS ALLOGRAFTS; SAPHENOUS-VEIN; RECONSTRUCTION; HOMOGRAFTS; ARTERIAL; GRAFTS;
Keywords:
VEIN ALLOGRAFT; VEIN AUTOGRAFT; GRAFT REJECTION; VEIN CRYOPRESERVATION; SCANNING ELECTRON MICROSCOPY; TRANSMISSION ELECTRON MICROSCOPY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
G. Pasquinelli et al., "ARE LONG-TERM CRYOPRESERVATION AND PATENCY OF VEIN ALLOGRAFT TRULY ACHIEVABLE", Cells and materials, 3(3), 1993, pp. 305-314

Abstract

Despite extensive experimental work, neither the effect of long term cryopreservation on vein graft architecture nor the failure of alloveins due to graft rejection have yet been investigated. Herein, we investigated ultrastructurally: a) the integrity of rabbit jugular veins following 1, 2 and 3 months of cryopreservation; b) the outcome of the three-month cryopreserved vein auto- and allografts after 1 month of implantation in the rabbit carotid artery; and c) the immunologic response to cryopreserved vein allografts with and without seeded autologousendothelium. Prior to implantation, the cryopreserved rabbit veins were well-maintained except for endothelial cell damage. Following implantation, the cryopreserved vein autografts were comparable to fresh veins with a complete endothelial lining. Conversely, only one of the allograft was still patent with features of acute rejection. After seeding with autologous endothelium, these explants failed shortly after surgery. We found absence of endothelium and necrosis of the media components with neutrophil infiltration. Although three months of cryopreservation does not affect vein graft architecture significantly, endothelial cells are damaged irrespective of the time of cryopreservation. Vein autografts promptly healed after one month of implantation at which time a viable endothelial cell lining was restored from the host artery. Conversely, vein allografts, with and without seeded autologous endothelium, failed due to graft rejection. This study highlights that current methods of cryopreservation do not reduce antigenicity of venous allografts significantly.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/08/20 alle ore 04:57:17