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Titolo:
ROLE FOR THE NA+ H+ EXCHANGER IN REPERFUSION STUNNING IN ISOLATED-PERFUSED RAT-HEART/
Autore:
DUTOIT EF; OPIE LH;
Indirizzi:
UNIV CAPE TOWN,SCH MED,HEART RES UNIT CAPE TOWN 7925 SOUTH AFRICA MRC CAPE TOWN SOUTH AFRICA
Titolo Testata:
Journal of cardiovascular pharmacology
fascicolo: 6, volume: 22, anno: 1993,
pagine: 877 - 883
SICI:
0160-2446(1993)22:6<877:RFTNHE>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOSOLIC FREE CALCIUM; FERRET HEARTS; VENTRICULAR ARRHYTHMIAS; ISCHEMIC-INJURY; H+ EXCHANGE; AMILORIDE; SODIUM; OVERLOAD; CELLS; CONTRACTION;
Keywords:
REPERFUSION STUNNING; ARRHYTHMIAS; NA+/H+ EXCHANGE; CALCIUM; AMILORIDE; HOE-694;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
E.F. Dutoit e L.H. Opie, "ROLE FOR THE NA+ H+ EXCHANGER IN REPERFUSION STUNNING IN ISOLATED-PERFUSED RAT-HEART/", Journal of cardiovascular pharmacology, 22(6), 1993, pp. 877-883

Abstract

We tested the hypothesis that Na+/H+ exchange contributes to reperfusion stunning and arrhythmias. The effects of amiloride, an establishedinhibitor of Na+/H+ exchange, were compared with those of a new inhibitor (HOE 694). Working hearts, subjected to 20-min global ischemia and reperfused for 30 min, were pretreated (for 5 min before ischemia) or reperfused (initial 2 min) with HOE 694 or amiloride. Pretreatment with 10(-7) M HOE increased recovery of aortic output (AO) after 30-minreperfusion: As a percentage of the preischemic controls, values were38.5 +/- 3.6% (n = 7) for controls versus 50.6 +/- 3.9% (n = 5) for the treated group (p < 0.05). Pretreatment with HOE (10(-6) M) increased AO recoveries from 38.2 +/- 2.0% (n = 5) to 52.9 +/- 2.7% (n = 5) (p< 0.05). Amiloride (10(-5) M) pretreatment improved AO recoveries from 41.6 +/- 2.7% (n = 6) to 55.8 +/- 4.0% (n = 6) (p < 0.05). Thus, pretreatment by both HOE 694 and amiloride decreased reperfusion stunning. Adding HOE (10(-7) M) only in the reperfusion period increased AO recoveries from 36.4 +/- 1.2% (n = 6) to 62.4 +/- 3.2% (n = 11) (p < 0.002). Amiloride (10(-5) or 10(-3) M) added only during reperfusion did not improve recovery of AO. HOE 694, active in much lower concentrations than amiloride, was the only compound active against stunning when added only during reperfusion. In addition, both compounds inhibited reperfusion arrhythmias when added at reperfusion. Hence, our data suggest that (a) HOE 694 is a more potent inhibitor of Na+/H+ exchange than amiloride; (b) inhibition of Na+/H+ exchange by HOE 694 even during reperfusion only limits reperfusion stunning; and (c) Na+/H+ exchange and, by implication, Na+/Ca2+ exchange during the early reperfusion period contributes to reperfusion stunning and arrhythmias in this model.

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Documento generato il 09/04/20 alle ore 18:28:51