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Titolo:
CORRELATIONS BETWEEN ISOCHORES AND CHROMOSOMAL BANDS IN THE HUMAN GENOME
Autore:
SACCONE S; DESARIO A; WIEGANT J; RAAP AK; DELLAVALLE G; BERNARDI G;
Indirizzi:
INST JACQUES MONOD,GENET MOLEC LAB,2 PL JUSSIEU F-75005 PARIS FRANCE INST JACQUES MONOD,GENET MOLEC LAB,2 PL JUSSIEU F-75005 PARIS FRANCE LEIDEN UNIV,DEPT CYTOCHEM & CYTOMETRY 2332 AL LEIDEN NETHERLANDS UNIV PAVIA,DIPARTIMENTO GENET & MICROBIOL A BUZZATITRAVERSO I-27100 PAVIA ITALY
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 24, volume: 90, anno: 1993,
pagine: 11929 - 11933
SICI:
0027-8424(1993)90:24<11929:CBIACB>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEQUENCE ORGANIZATION; INTERSPERSED REPEATS; TELOMERIC REGIONS; MAJOR COMPONENTS; CPG ISLANDS; MOUSE; VERTEBRATES; GENES;
Keywords:
IN-SITU HYBRIDIZATION; CHROMOSOME BANDING; COMPOSITIONAL MAP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
S. Saccone et al., "CORRELATIONS BETWEEN ISOCHORES AND CHROMOSOMAL BANDS IN THE HUMAN GENOME", Proceedings of the National Academy of Sciences of the United Statesof America, 90(24), 1993, pp. 11929-11933

Abstract

The human genome is made up of long DNA segments, the isochores, which are compositionally homogeneous and can be subdivided into a small number of families characterized by different G+C levels. Chromosome insitu suppression hybridization (in which excess unlabeled human DNA is added to suppress hybridization of repeated sequences present in theprobe, enabling enhanced observation of single-copy sequences) of DNAfractions characterized by an increasing G+C level was carried out todetermine the distribution of ''single-copy'' sequences correspondingto isochore families L1 + L2, H1, H2, and H3 on metaphase chromosomes. This produced a banding pattern progressing from a relatively diffuse staining to an R-banding, to a T-banding. More specifically, our results showed that (i) T-bands are formed by the G+C-richest isochores of the H3 family and by part of the G+C-rich isochores of the H1 and H2families (with a predominance of the latter); (ii) R'-bands (namely, R-bands exclusive of T-bands) are formed to almost equal extents by G+C-rich isochores of the H1 families (with a minor contribution of the H2 and H3 families) and by G+C-poor isochores of the L1 + L2 families;(iii) G-bands essentially consist of G+C-poor isochores from the L1 L2 families, with a minor contribution of isochores from the H1 family. These results not only clarify the correlations between DNA base composition and chromosomal bands but also provide information on the distribution of genes in chromosomes, gene concentration increasing withthe G+C levels of isochores.

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Documento generato il 28/11/20 alle ore 15:02:36