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Titolo:
TRANSGENIC MICE EXPRESSING HIGH PLASMA-CONCENTRATIONS OF HUMAN APOLIPOPROTEIN B100 AND LIPOPROTEIN(A)
Autore:
LINTON MF; FARESE RV; CHIESA G; GRASS DS; CHIN P; HAMMER RE; HOBBS HH; YOUNG SG;
Indirizzi:
UNIV CALIF SAN FRANCISCO,GLADSTONE INST CARDIOVASC DIS,POB 4191000 SAN FRANCISCO CA 94141 UNIV CALIF SAN FRANCISCO,CARDIOVASC RES INST SAN FRANCISCO CA 94141 UNIV CALIF SAN FRANCISCO,DEPT MED SAN FRANCISCO CA 94141 DNX BIOTHERAPEUT INC PRINCETON NJ 08540 UNIV TEXAS,SW MED CTR,DEPT MOLEC GENET & INTERNAL MED DALLAS TX 75235 UNIV TEXAS,SW MED CTR,DEPT BIOCHEM DALLAS TX 75235 UNIV TEXAS,SW MED CTR,HOWARD HUGHES MED INST DALLAS TX 75235 UNIV CALIF SAN FRANCISCO,GLADSTONE INST CARDIOVASC DIS,POB 4191000 SAN FRANCISCO CA 94141
Titolo Testata:
The Journal of clinical investigation
fascicolo: 6, volume: 92, anno: 1993,
pagine: 3029 - 3037
SICI:
0021-9738(1993)92:6<3029:TMEHPO>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY-LIPOPROTEIN; FAMILIAL DEFECTIVE APOLIPOPROTEIN-B-100; RECEPTOR-BINDING DOMAIN; MONOCLONAL-ANTIBODY; ATHEROSCLEROSIS SUSCEPTIBILITY; GENETIC-POLYMORPHISM; LDL RECEPTOR; HEPG2 CELLS; B BINDING; HYPERCHOLESTEROLEMIA;
Keywords:
P1 BACTERIOPHAGE; LOW DENSITY LIPOPROTEINS; CHOLESTEROL;
Tipo documento:
Note
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
M.F. Linton et al., "TRANSGENIC MICE EXPRESSING HIGH PLASMA-CONCENTRATIONS OF HUMAN APOLIPOPROTEIN B100 AND LIPOPROTEIN(A)", The Journal of clinical investigation, 92(6), 1993, pp. 3029-3037

Abstract

The B apolipoproteins, apo-B48 and apo-B100, are key structural proteins in those classes of lipoproteins considered to be atherogenic [e.g., chylomicron remnants, beta-VLDL, LDL, oxidized LDL, and Lp(a)]. Here we describe the development of transgenic mice expressing high levels of human apo-B48 and apo-B100. A 79.5-kb human genomic DNA fragment containing the entire human apo-B gene was isolated from a PI bacteriophage library and microinjected into fertilized mouse eggs. 16 transgenic founders expressing human apo-B were generated, and the animals with the highest expression had plasma apo-B100 levels nearly as high asthose of normolipidemic humans (approximately 50 mg/dl). The human apo-B100 in transgenic mouse plasma was present largely in lipoproteins of the LDL class as shown by agarose gel electrophoresis, chromatography on a Superose 6 column, and density gradient ultracentrifugation. When the human apo-B transgenic founders were crossed with transgenic mice expressing human apo(a), the offspring that expressed both transgenes had high plasma levels of human Lp(a). Both the human apo-B and Lp(a) transgenic mice will be valuable resources for studying apo-B metabolism and the role of apo-B and Lp(a) in atherosclerosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 06:09:30