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Titolo:
METHYLATION STATE OF THE HUMAN HLA-DRA GENE IN T-LYMPHOCYTES AND B-LYMPHOCYTES OF TRANSGENIC MICE - LACK OF METHYLATION AT ONE 5'-GCGC SITEIS NOT REQUIRED FOR GENE-EXPRESSION
Autore:
FERIOTTO G; POZZI L; CIUCCI A; CAMARDA G; MISCHIATI C; DAGNANO I; GAMBARI R; GIACOMINI P;
Indirizzi:
BIOCHEM INST,VIA L BORSARI I-44100 FERRARA ITALY UNIV FERRARA,IST CHIM BIOL I-44100 FERRARA ITALY UNIV ROMA LA SAPIENZA,DIPARTIMENTO BIOPATOL UMANA ROME ITALY IST REGINA ELENA,IMMUNOL LAB ROME ITALY CNR,IST TECNOL BIOMED ROME ITALY UNIV FERRARA,CTR INTERDIPARTIMENTALE BIOTECNOL I-44100 FERRARA ITALY CNR,IST REGINA ELENA,CHEMIOTERAPIA SPERIMENTALE PRECLIN LAB ROME ITALY
Titolo Testata:
European journal of biochemistry
fascicolo: 2, volume: 218, anno: 1993,
pagine: 485 - 492
SICI:
0014-2956(1993)218:2<485:MSOTHH>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAJOR HISTOCOMPATIBILITY COMPLEX; CLASS-II GENES; ALPHA-GENE; TRANSCRIPTIONAL REGULATION; CHROMATIN STRUCTURE; ANTIGENS; DETERMINANT; PATTERN; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
G. Feriotto et al., "METHYLATION STATE OF THE HUMAN HLA-DRA GENE IN T-LYMPHOCYTES AND B-LYMPHOCYTES OF TRANSGENIC MICE - LACK OF METHYLATION AT ONE 5'-GCGC SITEIS NOT REQUIRED FOR GENE-EXPRESSION", European journal of biochemistry, 218(2), 1993, pp. 485-492

Abstract

A consistent lack of DNA methylation at one or both of two GCGC (Hha I) restriction sites in the 5' region of the HLA-DRA gene has been previously documented by the use of methyl-sensitive restriction enzymes in human cells and tissues, irrespectively of their expression of DRalpha products. Evidence presently available, however, does not exclude that a lack of methylation in this region, although not sufficient, might be necessary for gene expression. In this report, we show that only one of the 5'-GCGC sites is protected, although less efficiently than in man, from CG-->(m)CG modifications in tissues and cells of transgenic mice carrying an expressed single copy of the HLA-DRA gene/diploid genome. We demonstrate that the two 5' GCGC sites of the HLA-DRA transgene are fully methylated in DRalpha- splenocytes (more than 80% T-lymphocytes), while one of them (the most 5' site) is not methylated ina fraction of DRalpha+ splenocytes (more than 95% B-lymphocytes). These results provide evidence that absence of DNA methylation in the 5' region is not necessary for, but might be associated with and possiblysecondary to the expression of the DRA gene.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/08/20 alle ore 00:13:26