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Titolo:
EUKARYOTIC INITIATION FACTOR-5A IS A CELLULAR TARGET OF THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REV ACTIVATION DOMAIN MEDIATING TRANSACTIVATION
Autore:
RUHL M; HIMMELSPACH M; BAHR GM; HAMMERSCHMID F; JAKSCHE H; WOLFF B; ASCHAUER H; FARRINGTON GK; PROBST H; BEVEC D; HAUBER J;
Indirizzi:
SANDOZ GMBH,RES INST,BRUNNER STR 59 A-1235 VIENNA AUSTRIA SANDOZ GMBH,RES INST,BRUNNER STR 59 A-1235 VIENNA AUSTRIA REPLIGEN CORP CAMBRIDGE MA 02139 EBERHARD KARLS UNIV TUBINGEN,INST PHYSIOL CHEM TUBINGEN GERMANY
Titolo Testata:
The Journal of cell biology
fascicolo: 6, volume: 123, anno: 1993,
parte:, 1
pagine: 1309 - 1320
SICI:
0021-9525(1993)123:6<1309:EIFIAC>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
STRUCTURAL GENE-EXPRESSION; VIRAL MESSENGER-RNA; HIV-1 REV; CHLORAMPHENICOL ACETYLTRANSFERASE; HYPUSINE MODIFICATION; VIRION EXPRESSION; RESPONSE ELEMENT; REGULATORY GENES; FACTOR EIF-4D; HTLV-III;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
77
Recensione:
Indirizzi per estratti:
Citazione:
M. Ruhl et al., "EUKARYOTIC INITIATION FACTOR-5A IS A CELLULAR TARGET OF THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REV ACTIVATION DOMAIN MEDIATING TRANSACTIVATION", The Journal of cell biology, 123(6), 1993, pp. 1309-1320

Abstract

Expression of human immunodeficiency virus type 1 (HIV-1) structural proteins requires the presence of the viral trans-activator protein Rev. Rev is localized in the nucleus and binds specifically to the Rev response element (RRE) sequence in viral RNA. Furthermore, the interaction of the Rev activation domain with a cellular cofactor is essentialfor Rev function in vivo. Using cross-linking experiments and Biospecific Interaction Analysis (BIA) we identify eukaryotic initiation factor 5A (eIF-5A) as a cellular factor binding specifically to the HIV-1 Rev activation domain. Indirect immunofluorescence studies demonstratethat a significant fraction of eIF-5A localizes to the nucleus. We also provide evidence that Rev trans-activation is functionally mediatedby eIF-5A in Xenopus oocytes. Furthermore, we are able to block Rev function in mammalian cells by antisense inhibition of eIF-5A gene expression. Thus, regulation of HIV-1 gene expression by Rev involves the targeting of RRE-containing RNA to components of the cellular translation initiation complex.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 14:21:33