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Titolo:
BARIUM PERMEABILITY OF NEURONAL NICOTINIC RECEPTOR-ALPHA-7 EXPRESSED IN XENOPUS-OOCYTES
Autore:
SANDS SB; COSTA ACS; PATRICK JW;
Indirizzi:
PFIZER INC,EASTERN POINT RD GROTON CT 06340 BAYLOR COLL MED,DIV NEUROSCI HOUSTON TX 77030
Titolo Testata:
Biophysical journal
fascicolo: 6, volume: 65, anno: 1993,
pagine: 2614 - 2621
SICI:
0006-3495(1993)65:6<2614:BPONNR>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; RAT CENTRAL NEURONS; ACETYLCHOLINE-RECEPTOR; SINGLE-CHANNEL; CALCIUM PERMEABILITY; VOLTAGE CLAMP; CONCENTRATION-DEPENDENCE; SYMPATHETIC NEURONS; ENDPLATE CHANNELS; DIVALENT-CATIONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
S.B. Sands et al., "BARIUM PERMEABILITY OF NEURONAL NICOTINIC RECEPTOR-ALPHA-7 EXPRESSED IN XENOPUS-OOCYTES", Biophysical journal, 65(6), 1993, pp. 2614-2621

Abstract

The rat alpha7 neuronal nicotinic acetylcholine receptor was expressed and studied in Xenopus oocytes. The magnitude and reversal potentialof instantaneous whole cell currents were examined in solutions containing varying concentrations of either calcium or barium, and in the presence or absence of the intracellular calcium chelator BAPTA. In external barium, application of nicotine elicits an inwardly rectifying response; in calcium the response is larger and has a linear IV relation. pretreatment of oocytes with BAPTA-AM could not prevent activation of calcium-dependent chloride channels in external Ringer containing calcium. Using an extended GHK equation, the permeability ratio P(Ba)/P(Na) of the alpha7 receptor was determined to be about 17. Our resultssuggest that alpha7 nicotinic receptors are highly permeable to divalent cations.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 22:10:17