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Titolo:
CLASSIFICATION OF CATECHOL 1,2-DIOXYGENASE FAMILY - SEQUENCE-ANALYSISOF A GENE FOR THE CATECHOL 1,2-DIOXYGENASE SHOWING HIGH SPECIFICITY FOR METHYLCATECHOLS FROM GRAM(-ASSIMILATING RHODOCOCCUS-ERYTHROPOLIS AN-13() ANILINE)
Autore:
MURAKAMI S; KODAMA N; SHINKE R; AOKI K;
Indirizzi:
KOBE UNIV,DEPT BIOFUNCT CHEM,NADA KU KOBE 657 JAPAN KOBE UNIV,GRAD SCH SCI & TECHNOL,DIV SCI BIOL RESOURCES,NADA KU KOBE 657 JAPAN
Titolo Testata:
Gene
fascicolo: 1, volume: 185, anno: 1997,
pagine: 49 - 54
SICI:
0378-1119(1997)185:1<49:COC1F->2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
PSEUDOMONAS-ARVILLA C-1; MICROBIAL-METABOLISM; AROMATIC-AMINES; ACINETOBACTER-CALCOACETICUS; PROTOCATECHUATE 3,4-DIOXYGENASE; EVOLUTIONARY DIVERGENCE; PUTIDA MT-2; PURIFICATION; PYROCATECHASE; ORGANIZATION;
Keywords:
ORTHO-CLEAVAGE; BETA-KETOADIPATE PATHWAY; CIS,CIS-MUCONATE; BIODEGRADATION; AROMATIC COMPOUND;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
S. Murakami et al., "CLASSIFICATION OF CATECHOL 1,2-DIOXYGENASE FAMILY - SEQUENCE-ANALYSISOF A GENE FOR THE CATECHOL 1,2-DIOXYGENASE SHOWING HIGH SPECIFICITY FOR METHYLCATECHOLS FROM GRAM(-ASSIMILATING RHODOCOCCUS-ERYTHROPOLIS AN-13() ANILINE)", Gene, 185(1), 1997, pp. 49-54

Abstract

Gram(+) aniline-assimilating Rhodococcus erythropolis AN-13 (AN-13) produces catechol 1,2-dioxygenase (C120) showing high enzymatic activities for 3- and 4-methylcatechols [Aoki et al. (1984) Agric. Biol. Chem. 48, 2087-2095]. A 3.0 kb Sau3AI fragment carrying a gene encoding C120 (catA) was cloned by selection of transformants showing C120 activity from a gene library of AN-13. Furthermore, we specified a 1.6 kb SalI fragment containing catA from the Sau3AI fragment by subcloning. Sequence analysis revealed that the 1.6 kb Sail fragment carried a 855 bp open reading frame (ORF) encoding the entire AN-13 catA, preceded bya potential ribosome binding site (RBS). From comparison of the deduced amino acid (aa) sequence of C120 from AN-13 with other C120 reported previously, it was found that the AN-13 enzyme shares 56.0% aa sequence identity with C120 from Arthrobacter sp. mA3 (mA3) [Eck and Belter(1991) Gene 123, 87-92] compared with less than 36.4% aa sequence identities with others. In conclusion, we classified all C120 including the AN-13 enzyme into three subfamilies on the basis of similarity of aa sequences, numbers of aa residues, and substrate specificity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 10:55:33