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Titolo:
IDENTIFICATION OF A SWEET-POTATO FEATHERY MOTTLE VIRUS ISOLATE FROM CHINA (SPFMV-CH) BY THE POLYMERASE CHAIN-REACTION WITH DEGENERATE PRIMERS
Autore:
COLINET D; KUMMERT J;
Indirizzi:
FAC SCI AGRONOM ETAT,PATHOL VEGETALE LAB B-5030 GEMBLOUX BELGIUM
Titolo Testata:
Journal of virological methods
fascicolo: 2, volume: 45, anno: 1993,
pagine: 149 - 159
SICI:
0166-0934(1993)45:2<149:IOASFM>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
COMPLETE NUCLEOTIDE-SEQUENCE; MOLECULAR-CLONING; CAPSID PROTEIN; GENOMIC RNA; POTYVIRUS; ACID;
Keywords:
SPFMV, SWEET POTATO FEATHERY MOTTLE VIRUS; POTYVIRUS; PCR; DEGENERATE PRIMER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
D. Colinet e J. Kummert, "IDENTIFICATION OF A SWEET-POTATO FEATHERY MOTTLE VIRUS ISOLATE FROM CHINA (SPFMV-CH) BY THE POLYMERASE CHAIN-REACTION WITH DEGENERATE PRIMERS", Journal of virological methods, 45(2), 1993, pp. 149-159

Abstract

Four degenerate oligonucleotide primers derived from conserved regions of the genome of potyviruses have been designed. A combined assay ofreverse transcription and the polymerase chain reaction utilizing these primers on:total RNA extracted from Ipomoea purpurea infected with a sweet potato feathery mottle virus isolate from China (SPFMV-CH), amplified a 1.35 kb and a 830 bp fragment. These amplified fragments were cloned into the Bluescript plasmid and sequenced. The comparison of the sequence of the N-terminal part of the coat protein of SPFMV-CH with those published for two other strains of SPFMV, showed a strong relationship between SPMV-CH and -RC.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 16:03:18