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Titolo:
PITUITARY ADENYLATE CYCLASE-ACTIVATING POLYPEPTIDE STIMULATES PROLACTIN GENE-EXPRESSION IN A RAT PITUITARY CELL-LINE
Autore:
COLEMAN DT; BANCROFT C;
Indirizzi:
CUNY MT SINAI SCH MED,DEPT PHYSIOL & BIOPHYS,1 GUSTAVE LEVY PL NEW YORK NY 10029 CUNY MT SINAI SCH MED,DEPT PHYSIOL & BIOPHYS,1 GUSTAVE LEVY PL NEW YORK NY 10029
Titolo Testata:
Endocrinology
fascicolo: 6, volume: 133, anno: 1993,
pagine: 2736 - 2742
SICI:
0013-7227(1993)133:6<2736:PACPSP>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
3',5'-CYCLIC ADENOSINE-MONOPHOSPHATE; VASOACTIVE INTESTINAL POLYPEPTIDE; HORMONE MESSENGER-RNA; HIGH-AFFINITY BINDING; GROWTH-HORMONE; CYCLIC-AMP; TRANSCRIPTION FACTOR; RESPONSIVE ELEMENT; SIGNALING PATHWAYS; FACTOR PIT-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
D.T. Coleman e C. Bancroft, "PITUITARY ADENYLATE CYCLASE-ACTIVATING POLYPEPTIDE STIMULATES PROLACTIN GENE-EXPRESSION IN A RAT PITUITARY CELL-LINE", Endocrinology, 133(6), 1993, pp. 2736-2742

Abstract

Pituitary adenylate cyclase-activating polypeptide (PACAP) has been shown to activate adenylate cyclase and stimulate PRL secretion in dispersed pituitary cells. We have employed the GH3 rat pituitary cell line to investigate whether PACAP can regulate expression of the PRL gene. PACAP increased cellular levels of cAMP in a concentration-dependentfashion (EC50, approximately 6 x 10(-9) m). PACAP also increased PRL mRNA levels in GH3 cells, implying that this peptide stimulates a stepin expression of the PRL gene. In addition, PACAP strongly stimulatedchloramphenicol acetyltransferase (CAT) activity in GH3 cells transiently transfected with a plasmid containing the first 187 basepairs of the rat PRL promoter cloned up-stream of the CAT gene, implying that PACAP stimulates transcription directed by the PRL promoter. The PACAP stimulation of CAT activity was observed at concentrations as low as 10(-11) M. We examined the action of PACAP on expression of a 5'-deletion series of PRL-CAT constructs. The PACAP response is completely lostwhen PRL Promoter sequences between positions -187 and -113 are removed, implying that neither a previously described sequence resembling acAMP response element nor the most proximal pit-1-binding site 1P plays a major role in the actions of PACAP on PRL gene transcription. This observation together with the ability of low concentrations of PACAPto stimulate PRL promoter activity without detectably increasing cellular cAMP levels suggest that the action of PACAP on PRL gene transcription might involve a cAMP-independent pathway.

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Documento generato il 23/11/20 alle ore 20:27:30