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Titolo:
PREPARATION OF PYRUVATE FROM (R)-LACTATE WITH PROTEUS SPECIES
Autore:
SCHINSCHEL C; SIMON H;
Indirizzi:
TECH UNIV MUNICH,LEHRSTUHL ORGAN CHEM & BIOCHEM,LICHTENBERGSTR 4 D-85747 GARCHING GERMANY TECH UNIV MUNICH,LEHRSTUHL ORGAN CHEM & BIOCHEM,LICHTENBERGSTR 4 D-85747 GARCHING GERMANY
Titolo Testata:
Journal of biotechnology
fascicolo: 2, volume: 31, anno: 1993,
pagine: 191 - 203
SICI:
0168-1656(1993)31:2<191:POPF(W>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHIRAL COMPOUNDS; REDUCTION; CELLS; ACIDS; CONVERSION; FORMATE;
Keywords:
(2R)-HYDROXYCARBOXYLATE-VIOLOGEN-OXIDOREDUCTASE; ELECTRO-ENZYMATIC OXIDATION; (R)-LACTATE DEHYDROGENATION; PROTEUS-VULGARIS; PROTEUS-MIRABILIS; PYRUVATE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
C. Schinschel e H. Simon, "PREPARATION OF PYRUVATE FROM (R)-LACTATE WITH PROTEUS SPECIES", Journal of biotechnology, 31(2), 1993, pp. 191-203

Abstract

Proteus vulgaris or P. mirabilis dehydrogenate (R)-lactate with high space time yield and high productivity numbers. Twenty grammes (dry weight) resting cells of P. mirabilis per litre converted a 0.65 M solution of (R)-lactate in 1 h to 94% pyruvate, which corresponds to a space time yield of 294 mol l-1 d-1 or about 25.8 kg l-1 d-1. In most cases anthraquinone-2,6-disulphonate was used as an effective redox mediator. The reduced quinone was regenerated by one of the following enzymic reductions: dimethylsulphoxide to dimethylsulphide, which leaves thesystem; N-oxides to amines; fumarate to succinate; and nitrate to nitrogen. For the latter mediator regeneration it was necessary to apply Paracoccus denitrificans together with Proteus species. The bioconversion of (R)-lactate to pyruvate can be conducted without using a buffer. Also the electrochemical reoxidation of anthraquinone-2,6-disulphonate is possible. Solutions of 0.24 M (R)-lactate can be dehydrogenated with a yield of 99% by stoichiometric quinone concentrations.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 16:43:22