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Titolo:
THE INFLUENCE OF 5'-SECONDARY STRUCTURES UPON RIBOSOME BINDING TO MESSENGER-RNA DURING TRANSLATION IN YEAST
Autore:
SAGLIOCCO FA; LASO MRV; ZHU DL; TUITE MF; MCCARTHY JEG; BROWN AJP;
Indirizzi:
UNIV ABERDEEN MARISCHAL COLL,DEPT MOLEC & CELL BIOL ABERDEEN AB9 1AS SCOTLAND UNIV ABERDEEN MARISCHAL COLL,DEPT MOLEC & CELL BIOL ABERDEEN AB9 1AS SCOTLAND GESELL BIOTECHNOL FORSCH MBH,DEPT GENE EXPRESS W-3300 BRAUNSCHWEIG GERMANY UNIV KENT,BIOL LAB CANTERBURY CT2 7NZ KENT ENGLAND
Titolo Testata:
The Journal of biological chemistry
fascicolo: 35, volume: 268, anno: 1993,
pagine: 26522 - 26530
SICI:
0021-9258(1993)268:35<26522:TIO5SU>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
SACCHAROMYCES-CEREVISIAE; EUKARYOTIC RIBOSOMES; SECONDARY STRUCTURE; CODON USAGE; 5' LEADER; INITIATION; SEQUENCE; GENE; EFFICIENCY; REGION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
F.A. Sagliocco et al., "THE INFLUENCE OF 5'-SECONDARY STRUCTURES UPON RIBOSOME BINDING TO MESSENGER-RNA DURING TRANSLATION IN YEAST", The Journal of biological chemistry, 268(35), 1993, pp. 26522-26530

Abstract

The influence of 5'-secondary structure formation and 5'-leader length upon mRNA translation in yeast has been analyzed using a closely related set of cat mRNAs (Vega Laso, M. R., Zhu, D., Sagliocco, F. A., Brown, A. J. P., Tuite, M. F., and McCarthy, J. E. G. (1993) J. Biol. Chem. 268, 6453-6462). A cat mRNA with a relatively short unstructured 5'-leader (22 bases) had a ribosome loading about half that of a cat mRNA with an unstructured 5'-leader of 77 bases. The introduction of 5'-secondary structures at various positions throughout the 5'-leader of the cat mRNA inhibited translation initiation, the degree of inhibition being largely dependent upon the thermodynamic stability of the structure. Each mRNA carrying a 5'-secondary structure had a biphasic polysome distribution, indicating that the mRNA molecules were distributedbetween untranslated and well translated subpopulations. This suggests that once 5'-secondary structures are unwound, they reform slowly relative to the rate of translation initiation in yeast. Untranslated mRNA accumulated in 43 S preinitiation complexes, even when there were only 5 bases between the 5'-cap and the base of the hairpin. The data are consistent with the scanning hypothesis (Kozak, M. (1989) J. Cell. Biol. 108, 229-241) and suggest that 40 S ribosomal subunits bind to mRNA early in the scanning process, probably before mRNA unwinding has taken place.

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Documento generato il 27/09/20 alle ore 18:48:30