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Titolo:
MONITORING OF CA2-TRANSIENTS IN ELECTRICALLY STIMULATED A7R5 VASCULARSMOOTH-MUSCLE CELLS FILLS THE EXPERIMENTAL GAP BETWEEN KCL-INDUCED DEPOLARIZATION AND PATCH-CLAMP STUDIES()
Autore:
KALTHOF B; BECHEM M; POTT L; SCHRAMM M;
Indirizzi:
BAYER AG,INST CARDIOVASC & ARTERIOSCLEROSIS RES D-42096 WUPPERTAL 1 GERMANY BAYER AG,INST CARDIOVASC & ARTERIOSCLEROSIS RES D-42096 WUPPERTAL 1 GERMANY RUHR UNIV BOCHUM,DEPT PHYSIOL W-4630 BOCHUM GERMANY
Titolo Testata:
Journal of pharmacological and toxicological methods
fascicolo: 3, volume: 30, anno: 1993,
pagine: 143 - 147
SICI:
1056-8719(1993)30:3<143:MOCIES>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHANNELS; LINES;
Keywords:
A7R5 CELLS; ELECTRICAL FIELD STIMULATION; CA2+-TRANSIENTS; VOLTAGE-DEPENDENT CA2+-CHANNELS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
10
Recensione:
Indirizzi per estratti:
Citazione:
B. Kalthof et al., "MONITORING OF CA2-TRANSIENTS IN ELECTRICALLY STIMULATED A7R5 VASCULARSMOOTH-MUSCLE CELLS FILLS THE EXPERIMENTAL GAP BETWEEN KCL-INDUCED DEPOLARIZATION AND PATCH-CLAMP STUDIES()", Journal of pharmacological and toxicological methods, 30(3), 1993, pp. 143-147

Abstract

The effects of electrical field stimulation on [Ca2+]i in the A7r5 vascular smooth muscle cell line have been monitored with the Ca2+-sensitive dye fura-2. The experimental set-up allowed high-temporal resolution of the [Ca2+]i-measurements and fast application of test solutions. Electrical field stimulation of A7r5 cells in the confluent growth state resulted in a transient increase in [Ca2+]i from resting values below 100 nM to values in the range of some hundred nM. For a given cell, the electrically induced Ca2+-transients were highly reproducible. The requirement for the presence of extracellular Ca2+ and the sensitivity to the Ca2+-antagonist nifedipine and the Ca2+-agonist BAY K 8644suggest that the Ca2+-transients reflect [Ca2+]i-changes based on Ca2-influx through voltage-dependent L-type Ca2+-channels. Therefore, electrical field stimulation of confluent A7r5 cells provides an easy-to-establish and highly reproducible method for the investigation of thephysiology and pharmacology of voltage-dependent Ca2+-channels in intact vascular smooth muscle cells, which fills the gap between KCl-induced depolarization and the patch-clamp technique.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 01:42:33