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Titolo:
ESTABLISHMENT OF CELL-LINES FROM B-CELL PRECURSOR ACUTE LYMPHOBLASTIC-LEUKEMIA
Autore:
ZHANG LQ; DOWNIE PA; GOODELL WR; MCCABE NR; LEBEAU MM; MORGAN R; SKLAR J; RAIMONDI SC; MILEY D; GOLDBERG A; LU MM; MONTAG A; SMITH SD;
Indirizzi:
UNIV CHICAGO,MED CTR,DEPT PEDIAT,5841 S MARYLAND AVE CHICAGO IL 60637 UNIV CHICAGO,MED CTR,DEPT PEDIAT,5841 S MARYLAND AVE CHICAGO IL 60637 UNIV CHICAGO,DEPT MED CHICAGO IL 60637 UNIV CHICAGO,DEPT PATHOL CHICAGO IL 60637 GENETRIX SCOTTSDALE AZ 00000 HARVARD UNIV,DEPT PATHOL BOSTON MA 02115 ST JUDE CHILDRENS HOSP,DEPT PATHOL & LAB MED MEMPHIS TN 38101
Titolo Testata:
Leukemia
fascicolo: 11, volume: 7, anno: 1993,
pagine: 1865 - 1874
SICI:
0887-6924(1993)7:11<1865:EOCFBP>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR REQUIREMENTS; T-CELL; PRE-B; REARRANGEMENT; LYMPHOMA; INVITRO; DIFFERENTIATION; TRANSLOCATIONS; PROLIFERATION; BREAKPOINT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
L.Q. Zhang et al., "ESTABLISHMENT OF CELL-LINES FROM B-CELL PRECURSOR ACUTE LYMPHOBLASTIC-LEUKEMIA", Leukemia, 7(11), 1993, pp. 1865-1874

Abstract

In an attempt to establish permanent cell lines from children with B-cell precursor acute lymphoblastic leukemia (BCP-ALL), 123 clinical samples from 117 patients were cultured in vitro. Using a method which was successful for the growth of ALL with T-cell phenotype, 3% (2174) of BCP-ALL samples from patients at diagnosis and 31% (9/29) of BCP-ALLsamples from patients at relapse were established as cell lines. However, in most cultures, leukemic cells survived for only a few weeks and the majority of viable cells present after 28 days of culture were esterase-positive mononuclear cells. Based on the hypothesis that mononuclear cells inhibited leukemic cell growth, we evaluated the effect of a monocyte toxin, L-leucine methyl ester (Leu-OMe), on the growth offour frozen BCP-ALL samples. Thawed leukemic cells treated with Leu-OMe, but not untreated control cells, proliferated in three samples andone new cell line was established. Subsequently, when Leu-OMe was added to fresh leukemia cells in culture, leukemic cell lines were grown from 29% (4/14) of samples at diagnosis and 66% (416) of relapse samples. Overall, 20 BCP-ALL cell lines were established, all were Epstein-Barr virus (EBV)-negative, and authenticity of each cell line was verified by a direct comparison of the immunophenotype, karyotype, and genotype with the patient's tumor cells. This improved method of cell culture permits a higher success rate of cell line establishment from patients with BCP-ALL thereby aiding in analysis of B-lymphocyte transformation and neoplasia.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 22:09:20