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Titolo:
THE EFFECT OF FASTING AND EXOGENOUS ADENOSINE ON ATP TISSUE CONCENTRATION AND VIABILITY OF CANINE PANCREAS GRAFTS DURING PRESERVATION BY THE 2-LAYER METHOD
Autore:
FUJINO Y; KURODA Y; MORITA A; TANIOKA Y; KU YS; SAITOH Y;
Indirizzi:
KOBE UNIV,SCH MED,DEPT SURG 1,CHUO KU,7-5-2 KUSUNOKI CHO KOBE 650 JAPAN KOBE UNIV,SCH MED,DEPT SURG 1,CHUO KU KOBE 650 JAPAN
Titolo Testata:
Transplantation
fascicolo: 5, volume: 56, anno: 1993,
pagine: 1083 - 1086
SICI:
0041-1337(1993)56:5<1083:TEOFAE>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLD-STORAGE METHOD; RETROGRADE OXYGEN PERSUFFLATION; DONOR NUTRITIONAL-STATUS; RAT-LIVER; TRANSPLANTATION; TRIPHOSPHATE; MECHANISM; NUCLEOTIDES; METABOLISM; ANALOGS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
Y. Fujino et al., "THE EFFECT OF FASTING AND EXOGENOUS ADENOSINE ON ATP TISSUE CONCENTRATION AND VIABILITY OF CANINE PANCREAS GRAFTS DURING PRESERVATION BY THE 2-LAYER METHOD", Transplantation, 56(5), 1993, pp. 1083-1086

Abstract

We have demonstrated a direct correlation between a high ATP level ina canine pancreas graft after preservation by the two-layer method and good posttransplant outcome. The purpose of this study was to examine the effect of fasting and exogenous adenosine on the ATP tissue level and viability of the canine pancreas graft during preservation by the two-layer method. Graft viability was judged by graft survival following autotransplantation. Maintenance of normoglycemia for 5 days post-transplant was considered graft survival. The pancreas was harvested from either 72-hr-fated (n=3) or -fed dogs (n=4) and preserved by the two-layer (Euro-Collins' solution [EC]/perfluorochemical [PFC]) methodfor 24 hr. Graft survival rates in fed and fasted groups were 4/4 (100%) and 3/3 (100%), respectively. There was no significant difference in ATP tissue concentrations between the two groups (7.48+/-0.55 vs. 7.03+/-0.74 mu mol/g dry wt, NS). The pancreas was subjected to 60 min warm ischemia and then was preserved by the two-layer method using EC or EC containing 5 mM adenosine for 24 hr. Without adenosine, graft survival rate was 0/3 (0%) and ATP tissue levels were not changed duringpreservation (1.62+/-0.26 vs. 1.56+/-0.40 mu mol/g dry wt, NS). However, provision of adenosine to the graft during preservation led to therestoration of ATP tissue levels (1.90+/-0.54 vs. 8.13+/-0.98 mu mol/g dry wt, P<0.01) in 4 of 5 grafts, and these grafts functioned immediately and maintained normoglycemia after transplantation. Graft survival rate was 4/5 (80%). One of 5 grafts, however, did not survive, and the ATP tissue level was not adequately recovered during preservation compared with viable grafts (3.67 vs. 8.13+/-0.98 mu mol/g dry wt). This study clearly demonstrates that the nutritional state of the donor has no influence on the ATP tissue level and viability of the graft during 24-hr preservation by the two-layer method. On the other hand, provision of adenosine to the graft during preservation stimulates ATP synthesis and improves the viability of the ischemically damaged pancreas.

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Documento generato il 08/04/20 alle ore 09:07:36