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Titolo:
C5A-INDUCED MYOCARDIAL-ISCHEMIA - ROLE FOR CD18-DEPENDENT PMN LOCALIZATION AND PMN-PLATELET INTERACTIONS
Autore:
FLETCHER MP; STAHL GL; LONGHURST JC;
Indirizzi:
UNIV CALIF DAVIS,SCH MED,DEPT INTERNAL MED,DIV RHEUMATOL ALLERGY & CLIN IMMUNOL,TB 192 DAVIS CA 95616 UNIV CALIF DAVIS,SCH MED,DEPT HUMAN PHYSIOL,DIV CARDIOVASC MED DAVIS CA 95616
Titolo Testata:
The American journal of physiology
fascicolo: 5, volume: 265, anno: 1993,
parte:, 2
pagine: 80001750 - 80001761
SICI:
0002-9513(1993)265:5<80001750:CM-RFC>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEUTROPHIL ACTIVATION; MONOCLONAL-ANTIBODY; LEUKOCYTE ADHESION; INTRACORONARY C5A; RABBIT PLATELETS; COMPLEMENT; INJURY; RECEPTOR; INVIVO; CHEMOATTRACTANTS;
Keywords:
ANAPHYLATOXIN; ADHERENCE; NEUTROPHILS; PLATELETS; THROMBOXANE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
M.P. Fletcher et al., "C5A-INDUCED MYOCARDIAL-ISCHEMIA - ROLE FOR CD18-DEPENDENT PMN LOCALIZATION AND PMN-PLATELET INTERACTIONS", The American journal of physiology, 265(5), 1993, pp. 80001750-80001761

Abstract

Intracoronary C5a in swine decreases coronary blood flow and regionalmyocardial segment shortening, responses mediated by thromboxane (Tx)A2-induced coronary vasoconstriction and intramyocardial trapping of granulocytes (PMNs). We sought to determine the origin of TxA2 and to investigate the role of CD18-dependent PMN function by utilizing an anti-CD18 monoclonal antibody, IB4. Isolated C5a-stimulated PMNs or platelets did not produce TxB2. However, together, C5a-stimulated PMNs andplatelets produced TxB2. IB4 bound porcine PMN surface CD18 and blocked C5a-induced PMN functions. In vivo, IB4 loading (2 mg/kg) transiently decreased arterial blood pressure and circulating platelet counts in six of nine animals (390 +/- 31 vs. 176 +/- 41 X 10(6)/ml, control vs. IB4; P < 0.002) and significantly ameliorated C5a-induced decreasesin coronary venous PMN count (-4.1 +/- 0.6 vs. -1.4 +/- 0.8 X 10(6) cells/ml), coronary artery blood flow (-10 +/- 1 vs. -4 +/- 1 ml/min), and segment shortening (-15 +/- 2 vs. -8 +/- 2%, C5a vs. C5a + IB4). We conclude that 1) production of TxB2 in response to C5a is mediated by a PMN-platelet interaction, 2) IB4 functionally blocks CD18 on porcine PMNs, and 3) C5a-induced myocardial PMN extraction is mediated, in part, by a CD18-dependent mechanism. These results suggest that PMN-platelet interactions and CD18-dependent PMN extraction are important inC5a-induced myocardial ischemia.

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Documento generato il 01/04/20 alle ore 02:07:02