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Titolo:
ARCHITECTURE OF THE MAIZE MITOCHONDRIAL ATP1 PROMOTER AS DETERMINED BY LINKER-SCANNING AND POINT MUTAGENESIS
Autore:
RAPP WD; LUPOLD DS; MACK S; STERN DB;
Indirizzi:
CORNELL UNIV,BOYCE THOMPSON INST PLANT RES ITHACA NY 14853 CORNELL UNIV,BOYCE THOMPSON INST PLANT RES ITHACA NY 14853
Titolo Testata:
Molecular and cellular biology
fascicolo: 12, volume: 13, anno: 1993,
pagine: 7232 - 7238
SICI:
0270-7306(1993)13:12<7232:AOTMMA>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTION INITIATION SITES; RNA-POLYMERASE; INVITRO TRANSCRIPTION; SPECIFICITY FACTOR; GENE-EXPRESSION; DNA; IDENTIFICATION; CHLOROPLASTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
W.D. Rapp et al., "ARCHITECTURE OF THE MAIZE MITOCHONDRIAL ATP1 PROMOTER AS DETERMINED BY LINKER-SCANNING AND POINT MUTAGENESIS", Molecular and cellular biology, 13(12), 1993, pp. 7232-7238

Abstract

Plant mitochondrial promoters are poorly conserved but generally share a loose consensus sequence spanning approximately 17 nucleotides. Using a homologous in vitro transcription system, we have previously shown that an 11-nucleotide sequence within this region comprises at least part of the maize mitochondrial atp1 promoter (W. Rapp and D. Stern,EMBO J. 11:1065-1073, 1992). We have extended this finding by using aseries of linker-scanning and point mutations to define the atp1 promoter in detail. Our results show that mutations at positions -12 to +5, relative to the major transcription start site, can decrease initiation rates to between < 10 and 40% of wild-type levels. Some mutations,scattered throughout this region, have lesser effects or no effect. Taken together, our data suggest a model in which the atp1 promoter consists of a central domain extending from -7 to +5 and an upstream domain of 1 to 3 bp that is centered around -11 to -12. Because many mutations within this promoter region are tolerated in vitro, the maize atp1 promoter is distinct from the highly conserved yeast mitochondrial promoters.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 07:19:41