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Titolo:
PROTEIN-PHOSPHORYLATION PATTERNS DURING IN-VITRO MATURATION OF THE GOAT OOCYTE
Autore:
GALL L; LEGAL F; DESMEDT V;
Indirizzi:
INRA,PHYSIOL ANIM STN,UNITE BIOL FECONDAT F-78325 JOUY EN JOSAS FRANCE
Titolo Testata:
Molecular reproduction and development
fascicolo: 4, volume: 36, anno: 1993,
pagine: 500 - 506
SICI:
1040-452X(1993)36:4<500:PPDIMO>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
GERMINAL VESICLE BREAKDOWN; MEIOTIC MATURATION; BOVINE OOCYTES; MOUSE OOCYTES; CELL-CYCLES; 6-DIMETHYLAMINOPURINE; ACTIVATION; KINASE; INHIBITORS; METAPHASE;
Keywords:
CAPRINE OOCYTE; MEIOTIC MATURATION; 6-DMAP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
L. Gall et al., "PROTEIN-PHOSPHORYLATION PATTERNS DURING IN-VITRO MATURATION OF THE GOAT OOCYTE", Molecular reproduction and development, 36(4), 1993, pp. 500-506

Abstract

Protein phosphorylation patterns were studied by radiolabelling goat cumulus oocyte complexes with [P-32] orthophosphate for various periods of time. The radiolabelled denuded oocytes were assessed for nuclearstatus and were used individually for gel electrophoresis. This studydemonstrated that specific changes in protein phosphorylations were programmed during goat oocyte maturation. One of the most prominent changes was a general increase in the phosphorylation rate at germinal vesicle breakdown (GVBD). From 8 hr of culture, dominant phosphoprotein bands with apparent molecular weights of 27, 31, 40, and 50 kD were observed; they remained at this level until the metaphase II stage. In the molecular weight range of 65-80 kD, the protein phosphorylation pattern exhibited characteristic differences, with a complex series of phosphoproteins appearing and disappearing, during maturation. Addition of 6-dimethylaminopurine (6-DMAP) at the onset of culture blocked the maturation process after GVBD and induced a dramatic condensation of chromatin. When added at different times after GVBD, 6-DMAP invariably induced chromosome condensation. This inhibition was partly reversible; i.e., after removal of the drug, oocytes were able to progress only until metaphase I. (C) 1993 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 04:02:56