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Titolo:
EVIDENCE THAT BIPHASIC EFFECTS OF BASIC FIBROBLAST GROWTH-FACTOR ON 5-ENE-3-BETA-HYDROXYSTEROID DEHYDROGENASE-ISOMERASE ACTIVITY IN CULTURED IMMATURE LEYDIG-CELLS ARE MEDIATED BY BINDING TO HEPARAN-SULFATE PROTEOGLYCANS
Autore:
MURONO EP; GOFORTH DP; WASHBURN AL; WU NX;
Indirizzi:
UNIV S CAROLINA,SCH MED,DEPT MED,ADM BLDG 28 COLUMBIA SC 29208 UNIV S CAROLINA,SCH MED,DEPT PHYSIOL COLUMBIA SC 29208 DORN VET ADM HOSP,RES SERV COLUMBIA SC 29208
Titolo Testata:
Journal of steroid biochemistry and molecular biology
fascicolo: 5, volume: 46, anno: 1993,
pagine: 557 - 563
SICI:
0960-0760(1993)46:5<557:ETBEOB>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMINOGEN-ACTIVATOR PRODUCTION; CAPILLARY ENDOTHELIAL-CELLS; 5-ALPHA-REDUCTASE ACTIVITY; EXTRACELLULAR-MATRIX; TESTOSTERONE; STIMULATION; STEROIDOGENESIS; RECEPTOR; TISSUES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
E.P. Murono et al., "EVIDENCE THAT BIPHASIC EFFECTS OF BASIC FIBROBLAST GROWTH-FACTOR ON 5-ENE-3-BETA-HYDROXYSTEROID DEHYDROGENASE-ISOMERASE ACTIVITY IN CULTURED IMMATURE LEYDIG-CELLS ARE MEDIATED BY BINDING TO HEPARAN-SULFATE PROTEOGLYCANS", Journal of steroid biochemistry and molecular biology, 46(5), 1993, pp. 557-563

Abstract

The present studies examined the effects of heparin, heparinases, insulin or insulin-like growth factor-I on basic fibroblast growth factoractions on 5-ene-3beta-hydroxy-steroid dehydrogenase-isomerase activity of cultured immature rat Leydig cells. Treatment with basic fibroblast growth factor alone (0.025-20 ng/ml) for 2 days had a biphasic effect on enzyme activity, with lower concentrations (0.025-1 ng/ml) progressively inhibiting activity to approximately 20% of control, while higher concentrations (2.5-20 ng/ml) partially reversed the inhibitive effects. The inclusion of 10 mug/ml heparin, a concentration reported to inhibit growth factor binding to heparan sulfate proteoglycans, blocked the increase in enzyme activity elicited by higher growth factor concentrations, but had no effect on the progressive decline in activity due to lower concentrations. Concomitant treatment with heparinase I and III, which specifically hydrolyze heparan sulfate proteoglycans,had a similar effect. In addition, both insulin and insulin-like growth factor-I partially reversed the inhibition of enzyme activity due to treatment with 1 ng/ml basic fibroblast growth factor. These studiessuggest that some basic fibroblast growth factor actions on cultured immature Leydig cells are mediated by binding to heparan sulfate proteoglycans, and that both insulin and insulin-like growth factor-I can reverse the inhibitive effects on 5-ene-3beta-hydroxysteroid dehydrogenase-isomerase activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 16:27:38