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Titolo:
ACETYLCHOLINE-RECEPTOR SUBUNIT GENE-EXPRESSION IN THYMIC TISSUE
Autore:
KAMINSKI HJ; FENSTERMAKER RA; ABDULKARIM FW; CLAYMAN J; RUFF RL;
Indirizzi:
CASE WESTERN RESERVE UNIV HOSP,SCH MED,CLEVELAND VET AFFAIRS MED CTR,DEPT NEUROL 127W CLEVELAND OH 44106 CASE WESTERN RESERVE UNIV HOSP,SCH MED,CLEVELAND VET AFFAIRS MED CTR,DEPT PATHOL CLEVELAND OH 44106 CASE WESTERN RESERVE UNIV HOSP,SCH MED,CLEVELAND VET AFFAIRS MED CTR,DEPT NEUROSURG CLEVELAND OH 44106 CASE WESTERN RESERVE UNIV HOSP,SCH MED,CLEVELAND VET AFFAIRS MED CTR,DEPT SURG CLEVELAND OH 44106
Titolo Testata:
Muscle & nerve
fascicolo: 12, volume: 16, anno: 1993,
pagine: 1332 - 1337
SICI:
0148-639X(1993)16:12<1332:ASGITT>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; MYASTHENIA-GRAVIS; ALPHA-SUBUNIT; GAMMA-SUBUNIT; CALF-MUSCLE; CELLS; TRANSCRIPTION; CDNA; AMPLIFICATION; THYMOMAS;
Keywords:
MYASTHENIA GRAVIS; ACETYLCHOLINE RECEPTOR; POLYMERASE CHAIN REACTION; MYF-4; MUSCLE DISEASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
H.J. Kaminski et al., "ACETYLCHOLINE-RECEPTOR SUBUNIT GENE-EXPRESSION IN THYMIC TISSUE", Muscle & nerve, 16(12), 1993, pp. 1332-1337

Abstract

It is controversial whether acetylcholine receptors (AChR) or AChR-like proteins exist in human thymus. To evaluate this question we isolated RNA from paraffin-embedded thymic tissue of 5 myasthenics and 5 nonmyasthenics. RNA was subjected to reverse transcription followed by polymerase chain reaction (RT-PCR) using primers specific for beta-actin, subunits of the fetal- and adult-type AChR, and Myf-4, a gene product which regulates AChR expression in muscle. Beta-Actin transcripts were identified in specimens from 8 of 10 patients. In these 8 patients alpha-and epsilon-Subunit transcripts were identified. Presence of these transcripts did not correlate with thymic pathology or clinical presentation. No gamma-subunit (specific for the fetal-type AChR) or Myf-4 transcripts were found. Our results indicate that mRNA for subunits of the adult-type AChR are expressed in thymic tissue by mechanisms not involving Myf-4 AChR subunits in pathological thymus may provide a target for immune attack in MG. However, as AChR subunits were found inthymus tissue from myasthenics and nonmyasthenics, the presence of AChR subunits in the thymus alone is not sufficient to produce myasthenia gravis. (C) 1993 John Wile & Sons Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 07:06:52