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Titolo:
CDNA CLONING AND EXPRESSION OF GUINEA-PIG NEUTROPHIL ATTRACTANT PROTEIN-1 (NAP-1) - NAP-1 IS HIGHLY CONSERVED IN GUINEA-PIG
Autore:
YOSHIMURA T; JOHNSON DG;
Indirizzi:
NCI,FCRDC,IMMUNOPATHOL SECT,IMMUNOBIOL LAB,BLDG 560,ROOM 12-71 FREDERICK MD 21702 PROGRAM RESOURCES INC DYN CORP,AIDS VACCINE PROGRAM,PROT CHEM SECT FREDERICK MD 21702
Titolo Testata:
The Journal of immunology
fascicolo: 11, volume: 151, anno: 1993,
pagine: 6225 - 6236
SICI:
0022-1767(1993)151:11<6225:CCAEOG>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOCYTE CHEMOATTRACTANT PROTEIN-1; MACROPHAGE INFLAMMATORY PROTEIN-2; BLOOD MONONUCLEAR LEUKOCYTES; AMINO-ACID-SEQUENCE; HUMAN INTERLEUKIN-8 RECEPTOR; GROWTH-STIMULATORY ACTIVITY; PERITONEAL-CAVITY INVIVO; CHEMOTACTIC FACTOR MDNCF; ACTIVATION PROTEIN-1; MOLECULAR-CLONING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
T. Yoshimura e D.G. Johnson, "CDNA CLONING AND EXPRESSION OF GUINEA-PIG NEUTROPHIL ATTRACTANT PROTEIN-1 (NAP-1) - NAP-1 IS HIGHLY CONSERVED IN GUINEA-PIG", The Journal of immunology, 151(11), 1993, pp. 6225-6236

Abstract

cDNA for neutrophil attractant protein-1 (NAP-1, also known as IL-8) was cloned from Con A-stimulated guinea pig spleen cells with human NAP-1 cDNA as a probe. Guinea pig NAP-1 cDNA is composed of 1433 bp withan open reading frame which encodes for a 101-amino-acid protein. Guinea pig NAP-1 had 70% amino acid sequence similarity to human NAP-1, which was much higher than a similarity between human and guinea pig monocyte chemoattractant protein-1 (MCP-1) (56%). Nucleotide sequence similarity within the coding region was 75%. To confirm its biological activity in guinea pig, recombinant guinea pig NAP-1 was expressed in COS-7 cells then purified. N-terminal sequence analysis gave two different N-termini at position 23 (Met) or 24 (Val). The two proteins showed their peak activity for guinea pig neutrophils at the concentration of 1 mug/ml (10(-7) M). Despite its high similarity to human NAP-1, the responsiveness of human neutrophils to guinea pig NAP-1 was minimum. Recombinant guinea pig NAP-1 caused strong neutrophil infiltration after intradermal injection into guinea pig skin. Since guinea pig is classified as a rodent, it was of interest to know whether human NAP-1 cDNA hybridizes to genomic DNA of other rodents such as mouse or rat, in which a NAP-1 homologue has not been found. Under low stringency conditions, human NAP-1 cDNA hybridized to human, rabbit, and guinea pig DNA, but not to mouse or rat DNA. Unlike NAP-1, human MCP-1 cDNA hybridized to genomic DNA of rabbit, guinea pig, mouse, and rat; MCP-1 cDNAhave been cloned from these species. The apparent absence of a NAP-1 gene in mouse or rat makes this chemoattractant unique among the members of the protein family to which NAP-1 and MCP-1 belong.

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Documento generato il 26/09/20 alle ore 13:42:25