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Titolo:
ESTROGEN REGULATION AND LOCALIZATION OF GALANIN GENE-EXPRESSION IN THE RAT UTERUS
Autore:
VRONTAKIS M; SCHROEDTER I; LEITE V; FRIESEN HG;
Indirizzi:
UNIV MANITOBA,DEPT PHYSIOL,770 BANNATYNE AVE WINNIPEG R3E 0W3 MANITOBA CANADA
Titolo Testata:
Biology of reproduction
fascicolo: 6, volume: 49, anno: 1993,
pagine: 1245 - 1250
SICI:
0006-3363(1993)49:6<1245:ERALOG>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPIDERMAL GROWTH-FACTOR; MESSENGER RIBONUCLEIC-ACID; ANTERIOR-PITUITARY; C-FOS; HYBRIDIZATION; RNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
M. Vrontakis et al., "ESTROGEN REGULATION AND LOCALIZATION OF GALANIN GENE-EXPRESSION IN THE RAT UTERUS", Biology of reproduction, 49(6), 1993, pp. 1245-1250

Abstract

To determine whether galanin is a target for estrogenic regulation inthe rat uterus, we measured the effects of estrogen on galanin mRNA expression in the uterus of ovariectomized rats and compared the results with the regulation of galanin in the anterior pituitary. Treatment of the animals with a single dose of 17beta-estradiol resulted in a rapid transient increase of galanin mRNA, similar to that in the pituitary of the same animals, with a peak at 3 h after stimulation and a return to prestimulation levels after 24 h. No galanin mRNA was detected in ovariectomized control animals in either tissue. By in situ hybridization of rat uterus 3 h after estrogen stimulation, we found that galanin mRNA was localized in the endometrium in stromal cells that are close to the lumen. There was no hybridization in the myometrium of theestrogen-treated animals. Surprisingly, and strikingly different fromresults in the pituitary, in the uterus with a constant and prolongedexposure to estrogen by diethylstilbestrol (DES) implants, the induction of galanin mRNA was only transient, with return to baseline levelsby 24 h despite the continued presence of DES. On the other hand, in the pituitary there was a rapid and sustained increase of galanin mRNA. These data demonstrate that 1) one of the initial steps in the mechanism of estrogen stimulation in the rat uterus involves galanin gene expression; 2) in the uterus galanin mRNA is expressed in stromal cellsof the endometrium; and 3) the regulation of galanin mRNA expression by estrogen in the pituitary and the uterus is markedly different.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 08:11:58