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Titolo:
MOLECULAR EPIDEMIOLOGY OF ESCHERICHIA-COLI O157-H7 STRAINS BY BACTERIOPHAGE-LAMBDA RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM ANALYSIS - APPLICATION TO A MULTISTATE FOODBORNE OUTBREAK AND A DAY-CARE-CENTER CLUSTER
Autore:
SAMADPOUR M; GRIMM LM; DESAI B; ALFI D; ONGERTH JE; TARR PI;
Indirizzi:
UNIV WASHINGTON,SCH PUBL HLTH & COMMUNITY MED,DEPT ENVIRONM HLTH SEATTLE WA 98195 CHILDRENS HOSP & MED CTR SEATTLE WA 98195 UNIV WASHINGTON,SCH MED,DEPT PEDIAT SEATTLE WA 98195
Titolo Testata:
Journal of clinical microbiology
fascicolo: 12, volume: 31, anno: 1993,
pagine: 3179 - 3183
SICI:
0095-1137(1993)31:12<3179:MEOEOS>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMOLYTIC UREMIC SYNDROME; HEMORRHAGIC COLITIS; INFECTIONS; 0157-H7; WASHINGTON; DIARRHEA; SEROTYPE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
M. Samadpour et al., "MOLECULAR EPIDEMIOLOGY OF ESCHERICHIA-COLI O157-H7 STRAINS BY BACTERIOPHAGE-LAMBDA RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM ANALYSIS - APPLICATION TO A MULTISTATE FOODBORNE OUTBREAK AND A DAY-CARE-CENTER CLUSTER", Journal of clinical microbiology, 31(12), 1993, pp. 3179-3183

Abstract

Genomic DNAs prepared from 168 isolates of Escherichia coli O157:H7 were analyzed for restriction fragment length polymorphisms on Southernblots probed with bacteriophage lambda DNA. The isolates analyzed included strains from a recent large multistate outbreak of E. coli 0157:H7 infection associated with consumption of poorly cooked beef in restaurants, a day-care center cluster, and temporally and geographically unrelated isolates. E. coli 0157:H7 isolates recovered from the incriminated meat and from 61 (96.8%) of 63 patients from Washington and Nevada possessed identical lambda restriction fragment length patterns. The lambda restriction fragment length polymorphisms observed in 11 (91.7%) of 12 day-care center patients were identical, but they differed from that of the strain associated with the multistate outbreak. E. coli 0157:H7 from 42 patients temporally or geographically unrelated to either cluster of infection possessed unique and different lambda restriction fragment length patterns, except for paired isolates from three separate clusters of infection. These data demonstrate that the hybridization of DNA digests of E. coli 0157:H7 with radiolabelled bacteriophage lambda DNA can be a useful, stable, and discriminatory epidemiologic tool for analyzing the linkage between strains of E. coli 0157:H7.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 05:30:58