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Titolo:
OVEREXPRESSION OF FULL-LENGTH OR PARTIAL-LENGTH MAP4 STABILIZES MICROTUBULES AND ALTERS CELL-GROWTH
Autore:
NGUYEN HL; CHARI S; GRUBER D; LUE CM; CHAPIN SJ; BULINSKI JC;
Indirizzi:
COLUMBIA UNIV COLL PHYS & SURG,DEPT PATHOL,630 W 168TH ST NEW YORK NY10032 COLUMBIA UNIV COLL PHYS & SURG,DEPT PATHOL NEW YORK NY 10032 COLUMBIA UNIV COLL PHYS & SURG,INTEGRATED PROGRAM CELLULAR MOL & BIOPHYS SCI NEW YORK NY 10032 COLUMBIA UNIV COLL PHYS & SURG,DEPT ANAT & CELL BIOL NEW YORK NY 10032
Titolo Testata:
Journal of Cell Science
, volume: 110, anno: 1997,
parte:, 2
pagine: 281 - 294
SICI:
0021-9533(1997)110:<281:OOFOPM>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
CULTURED MAMMALIAN-CELLS; DYNAMIC INSTABILITY; MITOTIC SPINDLES; BINDING DOMAINS; PROTEINS MAPS; ALPHA-TUBULIN; DNA-SYNTHESIS; LIVING CELLS; TAU-PROTEIN; EXPRESSION;
Keywords:
NOCODAZOLE; CELL CYCLE; DRUG RESISTANCE; DEXAMETHASONE; INDUCIBLE EXPRESSION; STABLE MICROTUBULE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
63
Recensione:
Indirizzi per estratti:
Citazione:
H.L. Nguyen et al., "OVEREXPRESSION OF FULL-LENGTH OR PARTIAL-LENGTH MAP4 STABILIZES MICROTUBULES AND ALTERS CELL-GROWTH", Journal of Cell Science, 110, 1997, pp. 281-294

Abstract

To investigate the in vivo functions of MAP4, a microtubule-associated protein expressed almost ubiquitously in vertebrate cells, we prepared stably transfected clonal mouse L(tk-) cell lines expressing full-length MAP4 (L-MAP4 cells) or its MT-binding domain (L-MTB cells). Although transfectants showed no dramatic defect in morphology, organellardistribution, or level of MT polymer, as compared to naive L(tk-) cells or L-MOCK cells (transfected with vector alone), MTs in L-MAP4 and L-MTB cells showed greater stability than those in control cells, as monitored by the level of post-translationally detyrosinated alpha-tubulin and by a quantitative nocodazole-resistance assay. In vivo, the MT-binding domain of MAP4 stabilized MTs less potently than full-length MAP4, in contrast to the equivalent efficacy demonstrated in studies of in vitro MT polymerization (Aizawa et al, (1991) J. Biol. Chem. 266,9841-9846). L-MAP4 and L-MTB cells grew significantly more slowly than control cells; this growth inhibition was not due to mitotic arrest or cell death, L-MAP4 and L-MTB cells also exhibited greater toleranceto the MT-depolymerizing agent, nocodazole, but not to the MT-polymerizing agent, Taxol. Our results demonstrate that MAP4 and its MT-binding domain are capable of MT stabilization in vivo, and that increasingthe intracellular level of MAP4 affects cell growth parameters.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 16:04:56