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Titolo:
HEMIN UPTAKE IN PORPHYROMONAS-GINGIVALIS - OMP26 IS A HEMIN-BINDING SURFACE PROTEIN
Autore:
BRAMANTI TE; HOLT SC;
Indirizzi:
UNIV TEXAS,HLTH SCI CTR,DEPT PERIODONT,7703 FLOYD CURL DR SAN ANTONIOTX 78284 UNIV TEXAS,HLTH SCI CTR,DEPT PERIODONT,7703 FLOYD CURL DR SAN ANTONIOTX 78284
Titolo Testata:
Journal of bacteriology
fascicolo: 22, volume: 175, anno: 1993,
pagine: 7413 - 7420
SICI:
0021-9193(1993)175:22<7413:HUIP-O>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
IRON TRANSPORT PROTEINS; GRAM-NEGATIVE BACTERIA; INFLUENZAE TYPE-B; OUTER-MEMBRANE; ESCHERICHIA-COLI; BACTEROIDES-GINGIVALIS; VIRULENCE; W50; PORPHYROMONAS-(BACTEROIDES)-GINGIVALIS; IDENTIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
T.E. Bramanti e S.C. Holt, "HEMIN UPTAKE IN PORPHYROMONAS-GINGIVALIS - OMP26 IS A HEMIN-BINDING SURFACE PROTEIN", Journal of bacteriology, 175(22), 1993, pp. 7413-7420

Abstract

A 26-kDa outer membrane protein (Omp26) has been proposed to play a role in hemin acquisition by Porphyromonas gingivalis (T. E. Bramanti and S. C. Holt, J. Bacteriol. 174:5827-5839, 1992). We studied [Fe-55]hemin uptake in P. gingivalis grown under conditions of hemin starvation (Omp26 expressed on the outer membrane surface) and hemin excess (Omp26 not expressed on surface). [Fe-55]hemin uptake occurred rapidly inhemin-starved cells which incorporated up to 70% of total [Fe-55]hemin within 3 min. P. gingivalis grown under hemin-starved conditions or treated with the iron chelator 2,2'-bipyridyl to induce an iron stresstook up six times more [Fe-55]hemin than hemin-excess-grown cells. Polyclonal monospecific anti-Omp26 antibody added to hemin-starved cellsinhibited [Fe-55]hemin uptake by more than 50%, whereas preimmune serum had no effect. [Fe-55]hemin uptake in hemin-starved P. gingivalis was inhibited (36 to 67%) in the presence of equimolar amounts of unlabeled hemin, protoporphyrin IX, zinc protoporphyrin, and Congo red dye but was not inhibited in the presence of non-hemin-containing iron sources. Heat shock treatment (45-degrees-C) of hemin-excess-grown P. gingivalis (which causes translocation of Omp26 to the surface) increased[Fe-55]hemin uptake by threefold after 3 min in comparison with cellsgrown at 37-degrees-C. However, no [Fe-55]hemin uptake beyond 3 min was observed in either hemin-excess-grown or hemin-starved cells exposed to heat shock. In experiments using heterobifunctional cross-linker analysis, hemin and selected porphyrins were cross-linked to Omp26 in hemin-starved P. gingivalis, but no cross-linking was seen with hemin-excess-grown cells. However, cross-linking of hemin to Omp26 was observed after heat shock treatment of hemin-excess-grown cells. Finally, anti-Omp26 antibody inhibited cross-linking of hemin to Omp26. These findings indicate that hemin binding and transport into the P. gingivalis cell is mediated by Omp26.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 09:57:46