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Titolo:
CONSTITUTIVE EXPRESSION OF TRANSGENIC HEAT-STABLE ANTIGEN (MCD24) IN LYMPHOCYTES CAN AUGMENT A SECONDARY ANTIBODY-RESPONSE
Autore:
NIELSEN PJ; EICHMANN K; KOHLER G; IGLESIAS A;
Indirizzi:
MAX PLANCK INST IMMUNBIOL,STUBEWEG 51 D-79108 FREIBURG GERMANY
Titolo Testata:
International immunology
fascicolo: 11, volume: 5, anno: 1993,
pagine: 1355 - 1364
SICI:
0953-8178(1993)5:11<1355:CEOTHA>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
T-CELL DEVELOPMENT; B-CELLS; SURFACE-ANTIGENS; GERM-LINE; DIFFERENTIATION; ADHESION; IMMUNOGLOBULIN; MECHANISMS; ACTIVATION; EMIGRANTS;
Keywords:
ACTIVATION; CD24; HEAT STABLE ANTIGEN; LYMPHOCYTE; T-CELL MATURATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
P.J. Nielsen et al., "CONSTITUTIVE EXPRESSION OF TRANSGENIC HEAT-STABLE ANTIGEN (MCD24) IN LYMPHOCYTES CAN AUGMENT A SECONDARY ANTIBODY-RESPONSE", International immunology, 5(11), 1993, pp. 1355-1364

Abstract

The murine heat stable antigen (HSA, mouse CD24) is a glycosyl phosphatidylinositol-anchored cell surface protein which is primarily expressed in immature but not mature cells of several hematopoietic lineagesand in neuronal tissue. The function of HSA is not known but there isevidence in lymphocytes that it is involved in cell adhesion and in cell activation. We examined the effect of constitutive HSA expression on the maturation and on the function of B and T cells in transgenic mice. Transgenic HSA was strongly expressed throughout all stages of T cell maturation without changes in absolute cell number or proportionsof subpopulations both in the thymus and in the periphery. The size of the B cell compartment was also unchanged. Thus, we conclude that inthe T lineage the loss of HSA expression is not mandatory for maturation. On the functional level, two parameters of immune function were measured. When the ability to activate peripheral T cells expressing transgenic HSA was tested in a mixed lymphocyte reaction, no significantdifference in the stimulation index and cytolytic activity was detected between transgenics and control littermates. However, when immunized with a T cell dependent antigen, transgenic mice showed 10-fold higher serum IgG1 titers. This suggests that the expression of transgenic HSA in cells normally negative for HSA (e.g. peripheral T cells or memory B cells) leads to a better stimulation of lymphocytes during a secondary antibody response.

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Documento generato il 05/07/20 alle ore 07:08:00