Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
INDIVIDUAL SUBUNITS OF BACTERIAL LUCIFERASE ARE MOLTEN GLOBULES AND INTERACT WITH MOLECULAR CHAPERONES
Autore:
FLYNN GC; BECKERS CJM; BAASE WA; DAHLQUIST FW;
Indirizzi:
UNIV OREGON,INST MOLEC BIOL EUGENE OR 97403 YALE UNIV,SCH MED,DEPT INTERNAL MED,INFECT DIS SECT NEW HAVEN CT 06510 UNIV OREGON,DEPT CHEM EUGENE OR 97403
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 22, volume: 90, anno: 1993,
pagine: 10826 - 10830
SICI:
0027-8424(1993)90:22<10826:ISOBLA>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID SEQUENCE; VIBRIO-HARVEYI; NUCLEOTIDE-SEQUENCE; STRUCTURAL CHARACTERIZATION; PROTEINS; GROEL; INTERMEDIATE; BINDING; GENE; NMR;
Keywords:
PROTEIN FOLDING; PROTEIN ASSEMBLY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
G.C. Flynn et al., "INDIVIDUAL SUBUNITS OF BACTERIAL LUCIFERASE ARE MOLTEN GLOBULES AND INTERACT WITH MOLECULAR CHAPERONES", Proceedings of the National Academy of Sciences of the United Statesof America, 90(22), 1993, pp. 10826-10830

Abstract

We have studied the assembly of a large heterodimeric protein, bacterial luciferase, by mixing purified subunits expressed separately in bacteria. The individual subunits alpha and beta contain much (66% and 50%, respectively) of the alpha-helix content of the native heterodimeras measured by circular dichroism, yet the alpha subunit lacks observable tertiary structure as measured by NMR. These results are consistent with the alpha subunit existing in a molten globule or collapsed form prior to assembly. The molecular chaperone GroEL binds reversibly to both subunits prior to assembly. Since these observations were obtained under physiological conditions, we propose that the molten globuleexists as a stable form during folding or assembly in the cell. Either the molten globule form of the subunits is an authentic folding intermediate or it is in rapid equilibrium with one. GroEL may function byfacilitating assembly through stabilization of these incompletely folded subunits.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 10:23:44