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Titolo:
KINETIC-PARAMETERS OF MONOVALENT CATION UPTAKE IN YEAST CALCULATED ONACCOUNTING FOR THE MUTUAL INTERACTION OF CATION UPTAKE AND MEMBRANE-POTENTIAL
Autore:
BORSTPAUWELS GWFH;
Indirizzi:
CATHOLIC UNIV NIJMEGEN,FAC SCI,CELL BIOL LAB 6525 ED NIJMEGEN NETHERLANDS
Titolo Testata:
Biochimica et biophysica acta
fascicolo: 2, volume: 1152, anno: 1993,
pagine: 201 - 206
SICI:
0006-3002(1993)1152:2<201:KOMCUI>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
SACCHAROMYCES-CEREVISIAE; PLASMA-MEMBRANE; POTASSIUM; PHOSPHATE; RB+; ACCUMULATION; ABSORPTION; TRANSPORT; K+;
Keywords:
MEMBRANE POTENTIAL; CATION TRANSPORT; TRANSPORT KINETICS; UPTAKE RATE; (YEAST); (SACCHAROMYCES-CEREVISIAE);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
G.W.F.H. Borstpauwels, "KINETIC-PARAMETERS OF MONOVALENT CATION UPTAKE IN YEAST CALCULATED ONACCOUNTING FOR THE MUTUAL INTERACTION OF CATION UPTAKE AND MEMBRANE-POTENTIAL", Biochimica et biophysica acta, 1152(2), 1993, pp. 201-206

Abstract

Kinetical parameters of monovalent cation uptake in yeast are calculated according to a model for mutual interaction of membrane potential and cation uptake. Apparent K(m) values for monovalent cation uptake obtained from uptake studies are 3-5-times lower than the K(m) values expected when the membrane potential remains constant at increasing cation concentrations instead of being reduced. The model accounts for various phenomena as (i) the increase in apparent K(m) of Rb+ uptake accompanying the decrease in maximum rate of uptake found on increasing the cellular K+ content, (ii) the decrease in maximum uptake rate and increase in K(m) in case of simultaneous transport of phosphate and Rb+, (iii) the change in the maximum uptake rate without a change in K(m)under conditions that the proton pump is effected and (iv) the absence of an increase in K+ efflux at high depolarizing external cation concentrations.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 13:18:20