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Titolo:
ENZYME ELECTRODE BASED ON OXALATE OXIDASE IMMOBILIZED IN GELATIN FOR SPECIFIC DETERMINATION OF OXALATE
Autore:
DINCKAYA E; TELEFONCU A;
Indirizzi:
EGE UNIV,FAK FEN,KIMYA BOLUMU,BIYOKIMYA ANABILIM DALI 35100 BORNOVA TURKEY
Titolo Testata:
Indian Journal of Biochemistry & Biophysics
fascicolo: 5, volume: 30, anno: 1993,
pagine: 282 - 284
SICI:
0301-1208(1993)30:5<282:EEBOOO>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
CONTINUOUS-FLOW SYSTEM; SPECTROPHOTOMETRIC DETERMINATION; URINARY OXALATE; HYPEROXALURIA; ABSORPTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
20
Recensione:
Indirizzi per estratti:
Citazione:
E. Dinckaya e A. Telefoncu, "ENZYME ELECTRODE BASED ON OXALATE OXIDASE IMMOBILIZED IN GELATIN FOR SPECIFIC DETERMINATION OF OXALATE", Indian Journal of Biochemistry & Biophysics, 30(5), 1993, pp. 282-284

Abstract

A biosensor for the specific determination of oxalate was developed using oxalate oxidase (EC 1.2.3.4) from barley (Hordeum vulgare) seedling roots in combination with a dissolved oxygen probe. Oxalate oxidaseimmobilized with gelatin using glutaraldehyde and fixed on pretreatedteflon membrane served as an enzyme electrode. The electrode responsewas maximum when 50 mM succinate buffer was used at pH 3.2 and 35-degrees-C. The biosensor response depends linearly on oxalate concentration between 5 x 10(-6) - 2 x 10(-4) M with response time 30 sec and substrate specificity of the oxalate oxidase electrode of 100%. The sensor is stable for more than 3 months during which time more than 400 assays can be performed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 19:00:59