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Titolo:
CORRECTION OF BILIRUBIN CONJUGATION IN THE GUNN RAT USING HEPATOCYTESIMMOBILIZED IN ALGINATE GEL BEADS AS AN EXTRACORPOREAL BIOARTIFICIAL LIVER
Autore:
FREMOND B; MALANDAIN C; GUYOMARD C; CHESNE C; GUILLOUZO A; CAMPION JP;
Indirizzi:
HOP PONTCHAILLOU,INSERM,U49,UNITE RECH HEPATOL F-35033 RENNES FRANCE BIOPREDIC F-35000 RENNES FRANCE HOP PONTCHAILLOU,CHIRURG INFANTILE CLIN F-35033 RENNES FRANCE HOP PONTCHAILLOU,CHIRURG CLIN B F-35033 RENNES FRANCE
Titolo Testata:
Cell transplantation
fascicolo: 6, volume: 2, anno: 1993,
pagine: 453 - 460
SICI:
0963-6897(1993)2:6<453:COBCIT>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
MICROCARRIER-ATTACHED HEPATOCYTES; HEPATIC-FAILURE; TRANSPLANTATION; SURVIVAL; CELLS;
Keywords:
IMMOBILIZED HEPATOCYTES; BIOARTIFICIAL LIVER; BILIRUBIN CONJUGATION; GUNN RAT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
B. Fremond et al., "CORRECTION OF BILIRUBIN CONJUGATION IN THE GUNN RAT USING HEPATOCYTESIMMOBILIZED IN ALGINATE GEL BEADS AS AN EXTRACORPOREAL BIOARTIFICIAL LIVER", Cell transplantation, 2(6), 1993, pp. 453-460

Abstract

A new extracorporeal bioartificial liver using alginate-entrapped hepatocytes was developed and evaluated for its ability to correct the lack of bilirubin conjugation in the Gunn rat. Hepatocytes were harvested from Sprague-Dawley rats by the two-step collagenase perfusion method and then immobilized in Ca++-alginate beads. The ability of immobilized hepatocytes to conjugate bilirubin was investigated in vitro by comparison with hepatocyte monolayer cultures. The bioartificial liver consisted of a cylindric bioreactor containing either alginate beads with hepatocytes (test group) or alginate beads alone (control group). Gunn rats were connected to this bioreactor via an extracorporeal circulation and bile fractions were collected at hourly intervals. Both bilirubin monoconjugates and bilirubin diconjugates were measured in the bile by high pressure liquid chromatography. Hepatocyte viability in alginate beads was determined prior to and at the end of each experiment and found to be unchanged (75%). In the test group, the concentration of bilirubin conjugates increased rapidly, attaining median values of 72.26 muM and 92.59 muM for mono and diconjugated bilirubin respectively, during a 3 h period of extracorporeal circulation. In the control group, the levels of either conjugate did not exceed 0.87 muM throughout the experiments. Statistical analysis showed a significant difference between the two groups (p < 0.0023). These results suggest that the bioartificial liver used in this study represents an effective method for the temporary correction of the Gunn rat's genetic defect. Sucha system might be of therapeutic interest in acute liver failure.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/21 alle ore 13:17:07