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Titolo:
EMBRYONIC AND FETAL-RAT MYOBLASTS EXPRESS DIFFERENT PHENOTYPES FOLLOWING DIFFERENTIATION IN-VITRO
Autore:
PIN CL; MERRIFIELD PA;
Indirizzi:
UNIV WESTERN ONTARIO,DEPT ANAT LONDON N6A 5C1 ONTARIO CANADA UNIV WESTERN ONTARIO,DEPT ANAT LONDON N6A 5C1 ONTARIO CANADA
Titolo Testata:
Developmental genetics
fascicolo: 5, volume: 14, anno: 1993,
pagine: 356 - 368
SICI:
0192-253X(1993)14:5<356:EAFMED>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAMMALIAN SKELETAL-MUSCLES; SECONDARY MYOTUBES; FIBER TYPES; MOUSE SOMITES; SLOW MYOSIN; CHAIN; HINDLIMB; CULTURES; CELLS; BUD;
Keywords:
MYOGENESIS; MYOSIN HEAVY CHAIN; RAT PRIMARY MUSCLE CULTURES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
C.L. Pin e P.A. Merrifield, "EMBRYONIC AND FETAL-RAT MYOBLASTS EXPRESS DIFFERENT PHENOTYPES FOLLOWING DIFFERENTIATION IN-VITRO", Developmental genetics, 14(5), 1993, pp. 356-368

Abstract

Myosin heavy chain (MHC) is encoded by a multigene family containing members which are expressed in developmental and fiber type-specific patterns. In developing rats, primary (1-degrees) and secondary (2-degrees) myotubes can be distinguished by differences in MHC expression: 1-degrees myotubes coexpress embryonic and slow MHC, while 2-degrees myotubes initially express only embryonic MHC. We have used monoclonal antibodies which recognize the embryonic, slow, neonatal, and adult fast IIB/IIX MHCs to examine MHC accumulation in myoblasts obtained from hindlimbs of embryonic day (ED) 14 and ED 20 Sprague-Dawley rats during differentiation in vitro. Embryonic myoblasts (ED 14), which developinto 1-degree myotubes in vivo, differentiate as myocytes or small myotubes (i.e., 1-4 nuclei) which express both embryonic and slow MHC. They do not accumulate detectable levels of neonatal or adult fast IIB/IIX MHC. Fetal myoblasts, which develop into secondary myotubes in vivo, fuse to form large myotubes (i.e., 10-50 nuclei) and express predominantly embryonic MHC at 3 days in culture. These myotubes accumulate neonatal and adult fast IIB/IIX isoforms of MHC and eventually contract spontaneously. In contrast to embryonic myotubes, they do not accumulate slow MHC. Our results demonstrate that embryonic and fetal rat myoblasts express different phenotypes in vitro and suggest that they represent distinct myoblast lineages similar to those previously described in chickens and mice. These two lineages may be responsible for thegeneration of distinct populations of 1-degree and 2-degrees myotubesin vivo. (C) 1993 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 20:59:14