Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ENHANCEMENT OF RETROVIRAL INFECTION IN-VITRO BY ANTI-LE(Y) IGG - REVERSAL BY HUMANIZATION OF MONOCLONAL MOUSE ANTIBODY
Autore:
HANSEN JES; SORENSEN AM; ARENDRUP M; OLOFSSON S; NIELSEN JO; JANZEK E; NIELSEN C; LOIBNER H;
Indirizzi:
HVIDOVRE UNIV HOSP,DEPT INFECT DIS 144 DK-2650 HVIDOVRE DENMARK STATENS SERUM INST,DEPT VIROL DK-2300 COPENHAGEN DENMARK SANDOZ GMBH VIENNA AUSTRIA GOTHENBURG UNIV,DEPT CLIN VIROL S-41124 GOTHENBURG SWEDEN
Titolo Testata:
APMIS. Acta pathologica, microbiologica et immunologica Scandinavica
fascicolo: 9, volume: 101, anno: 1993,
pagine: 711 - 718
SICI:
0903-4641(1993)101:9<711:EORIIB>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; HUMAN-ENDOTHELIAL CELLS; HIV-INFECTION; LEUKEMIA-VIRUS; TYPE-1; GLYCOSYLATION; INHIBITION; PEPTIDES; CANCER; SERUM;
Keywords:
HIV; HTLV-1; LE(Y); ENHANCEMENT; CARBOHYDRATE; IMMUNOTHERAPY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
J.E.S. Hansen et al., "ENHANCEMENT OF RETROVIRAL INFECTION IN-VITRO BY ANTI-LE(Y) IGG - REVERSAL BY HUMANIZATION OF MONOCLONAL MOUSE ANTIBODY", APMIS. Acta pathologica, microbiologica et immunologica Scandinavica, 101(9), 1993, pp. 711-718

Abstract

Monoclonal mouse IgG, antibody (ABL 364) against the carbohydrate Le(y) antigen enhanced infection in vitro with HTLV-1 and with HIV-1 whenpropagated in both transformed and normal lymphocytes. Enhancement was independent of complement, occurred with both lymphocytes and monocytes as target cells, and did not use either Le(y) epitopes on target cells for cross-linkage of virus to the cell or the Fc part of the antibody as a ligand for any cellular receptor. For enhancement to occur, binding of anti-Le(y) antibody to virus was required to take place before virus binding to its specific receptor with no indication of any alternative pathway of infection, as evidenced by abrogation of enhancement by anti-CD4 MAb or soluble recombinant CD4, and also the inability of anti-Le(y) MAb to mediate HIV infection of HSB-2 cells in which HTLV-1/HIV pseudovirus infection was enhanced. While F(ab)2 fragments of ABL 364 also enhanced infection, a human/mouse chimeric antibody anda fully humanized antibody had no enhancing effect on free virus infection. We suggest that binding of anti-Le(y) ABL 364 or its F(ab), fragment induced a conformational change in the gp120 oligomers facilitating the process of infection, and that this function was abrogated by the IgG1 Fc of the chimeric and the humanized antibodies. The observations indicate that the non-paratope domains of antiviral antibodies can influence their function as neutralizing or enhancing for infection.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 05:05:46