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Titolo:
BIOACTIVATION AND INACTIVATION OF AFLATOXIN B-1 BY HUMAN, MOUSE AND RAT-LIVER PREPARATIONS - EFFECT ON SCE IN HUMAN MONONUCLEAR LEUKOCYTES
Autore:
WILSON AS; WILLIAMS DP; DAVIS CD; TINGLE MD; PARK BK;
Indirizzi:
UNIV LIVERPOOL,DEPT PHARMACOL & THERAPEUT,POB 147 LIVERPOOL L69 3BX MERSEYSIDE ENGLAND UNIV LIVERPOOL,DEPT PHARMACOL & THERAPEUT LIVERPOOL L69 3BX MERSEYSIDE ENGLAND
Titolo Testata:
Mutation research
fascicolo: 2, volume: 373, anno: 1997,
pagine: 257 - 264
SICI:
0027-5107(1997)373:2<257:BAIOAB>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
S-TRANSFERASE-MU; IN-VITRO; DNA ADDUCTS; CANCER; CARCINOGEN; LYMPHOCYTE; EXPOSURE; DIETARY; TACRINE; EPOXIDE;
Keywords:
AFLATOXIN B-1; SCE; MONONUCLEAR LEUKOCYTE; CYP1A2; GST M1; EPOXIDE HYDROLASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
A.S. Wilson et al., "BIOACTIVATION AND INACTIVATION OF AFLATOXIN B-1 BY HUMAN, MOUSE AND RAT-LIVER PREPARATIONS - EFFECT ON SCE IN HUMAN MONONUCLEAR LEUKOCYTES", Mutation research, 373(2), 1997, pp. 257-264

Abstract

The purpose of this study was to investigate the use of human and animal subcellular liver fractions in an in vitro evaluation of carcinogenic risk. The bioactivation and bioinactivation of the known genotoxiccarcinogen aflatoxin B-1 by human, mouse and rat liver preparations was investigated using the SCE assay in human lymphocytes as a genotoxic endpoint. There was a 10-fold variation in SCE response (1.1-11.6 SCE/Cell) in human mononuclear leucocytes (MNLs) after aflatoxin B-1 wasactivated by human liver microsomes (n=6). Activation correlated withthe CYP1A2 phenotype of livers (r=0.8; p<0.05), but there was no correlation with either GST M1 genotype or epoxide hydrolase phenotype. Mouse liver microsomes activated aflatoxin B-1 to a greater extent [(1 mu M) 12.8+/-2.51 SCE/Cell] than either rat [(10 mu M) 12.0+/-3.84 SCE/Cell] or human (L25) [(10 mu M) 8.8+/-2.00 SCE/Cell] liver microsomes. The addition of mouse liver cytosol and reduced glutathione (GSH) significantly (p<0.001) reduced aflatoxin B-1-dependent genotoxicity, whereas the addition of either human or rat cytosol (+GSH) was without effect. These data indicate that species variation in both bioactivationand bioinactivation can exist. Therefore there is a necessity for careful selection of activation systems from species whose biochemical profile reflects that of man.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 22:02:33