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Titolo:
GLUTAMIC-ACID DECARBOXYLASE GENE-EXPRESSION IN THALAMIC RETICULAR NEURONS TRANSPLANTED AS A CELL-SUSPENSION IN THE ADULT THALAMUS
Autore:
NOTHIAS F; SALIN P; PESCHANSKI M; CHESSELET MF;
Indirizzi:
UNIV PENN,DEPT PHARMACOL,36TH ST & HAMILTON WALK PHILADELPHIA PA 19104 UNIV PENN,DEPT PHARMACOL,36TH ST & HAMILTON WALK PHILADELPHIA PA 19104 FAC MED CRETEIL,INSERM,CJF 91-02 F-94010 CRETEIL FRANCE
Titolo Testata:
Molecular brain research
fascicolo: 3, volume: 20, anno: 1993,
pagine: 245 - 253
SICI:
0169-328X(1993)20:3<245:GDGITR>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEUROTRANSMITTER MESSENGER-RNAS; STRIATAL GRAFTS; NIGROSTRIATAL LESIONS; INSITU HYBRIDIZATION; RAT; ORGANIZATION; NUCLEUS; BRAIN; LOCALIZATION; PHENOTYPE;
Keywords:
GLUTAMIC ACID DECARBOXYLASE MESSENGER RNA; IN-SITU HYBRIDIZATION HISTOCHEMISTRY; GRAFT; GABA; DEVELOPMENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
F. Nothias et al., "GLUTAMIC-ACID DECARBOXYLASE GENE-EXPRESSION IN THALAMIC RETICULAR NEURONS TRANSPLANTED AS A CELL-SUSPENSION IN THE ADULT THALAMUS", Molecular brain research, 20(3), 1993, pp. 245-253

Abstract

The goal of the present study was to determine whether alterations inneuronal morphology and connections in thalamic grafts were accompanied by changes in the expression of mRNA encoding glutamic acid decarboxylase (GAD), the key enzyme in the synthesis of GABA, the normal neurotransmitter of neurons of the thalamic reticular nucleus. Cell suspensions of rat fetal tissue containing both thalamic reticular nucleus and ventrobasal primordia were transplanted into the excitotoxically lesioned somatosensory thalamus of adult rats. Levels of messenger RNA (mRNA) encoding GAD (Mr 67,000; GAD67) were measured 7 days to 4 monthsfollowing transplantation via quantitative in situ hybridization withS-35-radiolabeled antisense RNAs. Expression of GAD67 mRNA in the thalamic reticular nucleus was analyzed in parallel in rat pups between 0and 30 days postnatally, and in adult animals. As already observed with immunohistochemistry, transplanted neurons of the thalamic reticular nucleus did not group in specific clusters but rather mingled with unlabeled (putatively ventrobasal) neurons. Levels of labelling for GAD67 mRNA per neuron increased over time and reached adult levels duringthe third week post-grafting, i.e. 2 weeks after the theoretical birthdate of the neurons (grafted at embryonic days 15-16). Similar valueswere observed and a plateau was reached at similar time points duringnormal ontogeny. The results suggest that, in contrast to morphology and size of the neuronal cell bodies, gene expression of GAD67 develops normally despite the ectopic location of neurons of the thalamic reticular nucleus in the somatosensory thalamus, the abnormal connectivity and the lack of segregation from non-GABAergic neurons. Therefore, dissociation and transplantation of the tissue, resulting in disruptionof the extracellular matrix and alteration of cellular interactions, differentially affects various aspects of neuronal maturation. Becauseneuronal activity in the graft is similar to that in the adult thalamic reticular nucleus, the results further suggest that, as shown in other experimental systems, GAD67 mRNA levels in the graft are regulatedin relation to the functional activity of GABAergic neurons.

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Documento generato il 13/07/20 alle ore 17:34:26