Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
REGIONAL DISTRIBUTION OF AMYLOID BETA-PROTEIN PRECURSOR, GROWTH-ASSOCIATED PHOSPHOPROTEIN-43 AND MICROTUBULE-ASSOCIATED PROTEIN-2 MESSENGER-RNAS IN THE NIGROSTRIATAL SYSTEM OF NORMAL AND WEAVER MUTANT MICE ANDEFFECTS OF VENTRAL MESENCEPHALIC GRAFTS
Autore:
SOLA C; MENGOD G; LOW WC; NORTON J; GHETTI B; PALACIOS JM; TRIARHOU LC;
Indirizzi:
INDIANA UNIV,SCH MED,DEPT PATHOL & LAB MED,CELLULAR & MOLEC NEUROPATHOL LAB,MED SCI BLDG A-142 INDIANAPOLIS IN 46202 INDIANA UNIV,SCH MED,DEPT PATHOL & LAB MED,CELLULAR & MOLEC NEUROPATHOL LAB,MED SCI BLDG A-142 INDIANAPOLIS IN 46202 SANDOZ PHARMA AG,PRECLIN RES CH-4002 BASEL SWITZERLAND CSIC,CTR INVEST & DESARROLLO E-08034 BARCELONA SPAIN UNIV MINNESOTA,SCH MED,DEPT NEUROSURG MINNEAPOLIS MN 55455 UNIV MINNESOTA,SCH MED,DEPT PHYSIOL MINNEAPOLIS MN 55455 UNIV MINNESOTA,SCH MED,PROGRAM NEUROSCI MINNEAPOLIS MN 55455 INDIANA UNIV,SCH MED,DEPT PSYCHIAT INDIANAPOLIS IN 46202 INDIANA UNIV,SCH MED,DEPT MED & MOLEC GENET INDIANAPOLIS IN 46202
Titolo Testata:
European journal of neuroscience
fascicolo: 11, volume: 5, anno: 1993,
pagine: 1442 - 1454
SICI:
0953-816X(1993)5:11<1442:RDOABP>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALZHEIMERS-DISEASE; INSITU HYBRIDIZATION; MOUSE HOMOLOG; CHROMOSOMAL LOCALIZATION; DIFFERENTIAL EXPRESSION; DOPAMINE DEFICIENCY; COMPLEMENTARY-DNA; CELL-SUSPENSIONS; CEREBRAL-CORTEX; GENETIC-LINKAGE;
Keywords:
GENE EXPRESSION; NEUROLOGICAL MUTANT MICE; SUBSTANTIA-NIGRA; NEURAL TRANSPLANT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
74
Recensione:
Indirizzi per estratti:
Citazione:
C. Sola et al., "REGIONAL DISTRIBUTION OF AMYLOID BETA-PROTEIN PRECURSOR, GROWTH-ASSOCIATED PHOSPHOPROTEIN-43 AND MICROTUBULE-ASSOCIATED PROTEIN-2 MESSENGER-RNAS IN THE NIGROSTRIATAL SYSTEM OF NORMAL AND WEAVER MUTANT MICE ANDEFFECTS OF VENTRAL MESENCEPHALIC GRAFTS", European journal of neuroscience, 5(11), 1993, pp. 1442-1454

Abstract

Using in situ hybridization histochemistry with [P-32]oligonucleotideprobes, we studied the cellular localization of RNA transcripts for amyloid beta-protein precursor (betaAPP), growth-associated phosphoprotein-43 (GAP-43) and microtubule-associated protein 2 (MAP2) in the mesostriatal system of normal (+/+) and weaver (wv/wv) mutant mice, whichlose mesencephalic dopamine neurons. In addition, expression of the same messages was studied in ventral mesencephalic cell suspensions transplanted to the weaver striatum. Transcripts encoding GAP-43, MAP2 and isoforms betaAPP695, betaAPP714 and betaAPP751 were present in normal substantia nigra and progressively reduced in weaver substantia nigra; such a reduction was correlated with dopamine neuron loss. The survival of dopamine neurons in unilateral intrastriatal grafts was documented by methamphetamine-induced rotational asymmetry tests and by tyrosine hydroxylase immunocytochemistry. High hybridization signals were obtained for GAP-43, MAP2, betaAPP695, betaAPP714 and betaAPP751 RNA transcripts in the grafted tissue; the betaAPP770 species-normally seenin striatum and not substantia nigra-was not expressed in the grafts,but it was present in the recipient striatum. Following immunocytochemical labelling with antibodies, GAP-43 and MAP2 immunoreactivities were seen in cell processes in the grafts and surrounding tissue, whereas betaAPP immunoreactivity was mainly found in grafted cell bodies. These results suggest that the transplanted mesencephalic cells mature very similarly to those in the normal substantia nigra, expressing different mRNAs that are normally present in the ventral midbrain and which are reduced in the weaver mutant as a consequence of dopamine neuronloss.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 13:07:58