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Titolo:
AGE-ASSOCIATED CHANGES IN BINDING OF HUMAN B-LYMPHOCYTES TO A V(H)3-RESTRICTED UNCONVENTIONAL BACTERIAL-ANTIGEN
Autore:
SILVERMAN GJ; SASANO M; WORMSLEY SB;
Indirizzi:
UNIV CALIF SAN DIEGO,DEPT MED 0663,9500 GILMAN DR LA JOLLA CA 92093 UNIV CALIF SAN DIEGO,SAM & ROSE STEIN INST RES AGING LA JOLLA CA 92093 UNIV CALIF SAN DIEGO,THEODORE GILDRED CANC CTR LA JOLLA CA 92093 CYTOMETRY ASSOCIATES SAN DIEGO CA 92121
Titolo Testata:
The Journal of immunology
fascicolo: 10, volume: 151, anno: 1993,
pagine: 5840 - 5855
SICI:
0022-1767(1993)151:10<5840:ACIBOH>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-A; GERMINAL-CENTERS; VH-GENE; STAPHYLOCOCCUS-AUREUS; HUMAN CD5+; POLYREACTIVE ANTIBODIES; IMMUNOGLOBULIN GENES; INSITU HYBRIDIZATION; RHEUMATOID FACTORS; IMMUNE-RESPONSE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
64
Recensione:
Indirizzi per estratti:
Citazione:
G.J. Silverman et al., "AGE-ASSOCIATED CHANGES IN BINDING OF HUMAN B-LYMPHOCYTES TO A V(H)3-RESTRICTED UNCONVENTIONAL BACTERIAL-ANTIGEN", The Journal of immunology, 151(10), 1993, pp. 5840-5855

Abstract

We have recently demonstrated that there is a site on Staphylococcal protein A (SpA) that interacts with B cell Ig receptors in a manner comparable with known T cell superantigens, because this binding specificity is restricted to Fab with V(H)3 H chains and most V(H)3 Ig bind SpA. In the present studies, SpA was used as a phenotypic marker for V(H)3 expression by human lymphoid cells. As expected, this Fab-mediatedbinding specificity was completely inhibited by certain V(H)3 antibodies but not by antibodies from other V(H) families. In multiparameter flow cytometric analyses, this binding activity was demonstrated to behighly prevalent among B cells (14 to 54%), and was more common amongIgM-bearing B cells compared with IgG-bearing B cells. In all studies, Fab-mediated binding of SpA was uniformly expressed by a greater proportion of CD5-positive B cells than CD5-negative B cells. The proportion of B lymphocytes with this V(H)3-restricted binding capacity was found to undergo age-associated changes, because a large proportion of the peripheral B cells of neonates (mean +/- SD, 46.0 +/- 2.9%) bind this site, but two 10-mo-old subjects and older children had significantly lower binding levels (29.0 +/- 3.5%) that were the same as bindinglevels by adult peripheral B lymphocytes (30.2 +/- 3.3%). In immunohistochemical studies, tonsilar B cells that bind this site on SpA were shown to be common in mantle zones and germinal centers of secondary follicles. We speculate that Fab-mediated SpA binding represents a fundamental and primitive binding capacity that is part of the human preimmune repertoire, and we discuss the implications for the observed age-dependent shift in Fab-mediated binding of SpA by peripheral blood B cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 21:46:22