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Titolo:
SEROTONIN ACTIVATION OF A CYCLIC-AMP-DEPENDENT SODIUM CURRENT IN AN IDENTIFIED NEURON FROM HELISOMA-TRIVOLVIS
Autore:
PRICE CJ; GOLDBERG JI;
Indirizzi:
UNIV ALBERTA,DEPT ZOOL,CW312 BIOL SCI CTR EDMONTON T6G 2E9 ALBERTA CANADA
Titolo Testata:
The Journal of neuroscience
fascicolo: 11, volume: 13, anno: 1993,
pagine: 4979 - 4987
SICI:
0270-6474(1993)13:11<4979:SAOACS>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH CONE MOTILITY; NEURITE OUTGROWTH; INTRACELLULAR CALCIUM; MOLLUSCAN NEURONS; SENSORY NEURONS; NERVOUS-SYSTEM; IONIC CURRENTS; INWARD CURRENT; RETINAL RODS; PATCH-CLAMP;
Keywords:
MOLLUSCAN ELECTROPHYSIOLOGY; ION CHANNEL; 5-HT PHARMACOLOGY; 2ND MESSENGERS; NEURONAL REGENERATION; NEURONAL DEVELOPMENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
C.J. Price e J.I. Goldberg, "SEROTONIN ACTIVATION OF A CYCLIC-AMP-DEPENDENT SODIUM CURRENT IN AN IDENTIFIED NEURON FROM HELISOMA-TRIVOLVIS", The Journal of neuroscience, 13(11), 1993, pp. 4979-4987

Abstract

The mechanisms by which neurotransmitters regulate neurite extension and growth cone motility have been extensively studied using identified Helisoma neurons regenerating in cell culture. Specific neurons, such as buccal neuron B19, display a complex response to the addition of 5-HT involving the generation of action potentials, influx of extracellular calcium, and cessation of neurite extension and growth cone motility. While several studies have addressed the role of calcium in thiscascade, little is known about the mechanism underlying the 5-HT-induced excitation of neuron B19. Therefore, we have begun to characterizethe ion currents, receptors, and second messengers involved in the 5-HT-dependent depolarization of B19. Exposure of B19 to 5-HT resulted in the activation of a maintained inward current. Ion substitution experiments revealed that this current was carried mainly by sodium ions. The use of 8-bromo-cAMP, forskolin, or the phosphodiesterase inhibitorisobutyl methylxanthine (IBMX) to increase intracellular cAMP levels all resulted in inward current activation in the absence of 5-HT. Moreover, preloading the neuron with 8-bromo-cAMP was sufficient to prevent further current activation by 5-HT. In addition, the IBMX-activated current was greatly enhanced when induced in the presence of 5-HT. Protein kinase inhibitors failed to prevent 5-HT activation of sodium current, suggesting that cAMP may directly activate the current, independent of phosphorylation. Pharmacological experiments showed the B19 5-HT receptor has an EC50 of approximately 10(-7) m and can be activated by various indole analogs of 5-HT. Furthermore, methysergide displayedpartial agonist activity. These results have identified receptors andion currents that may play crucial roles in the regulation of nervoussystem development during embryogenesis in Helisoma.

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Documento generato il 21/09/20 alle ore 05:06:05