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Titolo:
SYNTHESIS OF A TRITIATED HUMAN GROWTH-HORMONE RELEASING PEPTIDE
Autore:
SHU AYL; HEYS JR;
Indirizzi:
SMITHKLINE BEECHAM PHARMACEUT,DEPT SYNTHET CHEM,RADIOCHEM SECT UW2830,POB 1539 KING OF PRUSSIA PA 19406
Titolo Testata:
International journal of peptide & protein research
fascicolo: 5, volume: 42, anno: 1993,
pagine: 432 - 439
SICI:
0367-8377(1993)42:5<432:SOATHG>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
PITUITARY; HEXAPEPTIDE; INVITRO; RAT;
Keywords:
5,7-DIBROMOTRYPTOPHAN; EPIMERIC PEPTIDE SEPARATION; LABELED HUMAN GROWTH HORMONE RELEASING PEPTIDE; TRITIUM-HALOGEN EXCHANGE; TRITIUM MAGNETIC RESONANCE SPECTROSCOPY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
A.Y.L. Shu e J.R. Heys, "SYNTHESIS OF A TRITIATED HUMAN GROWTH-HORMONE RELEASING PEPTIDE", International journal of peptide & protein research, 42(5), 1993, pp. 432-439

Abstract

Tritium-labeled growth hormone releasing peptide His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 was synthesized by tritium-halogen exchange on the precursor His-5,7-Br2-D-Trp-Ala-Trp-D-Phe-Lys-NH2. The radiolabeled peptide had a specific activity of 29 Ci/mmol and a radiochemical purity of 95 %. The tritium label was shown by H-3 NMR to be located mostly at the expected 5,7-positions of the indole nucleus in the D-Trp residue. Thedibromopeptide was prepared by solid-phase peptide synthesis, employing racemic 5,7-Br2-Trp as a building block and separation of the resulting epimeric mixture by HPLC. 5,7-Br2-Trp was prepared by a five-stepsequence beginning with 2,4-dibromoaniline. The use of anisole as an additive in the HF resin/peptide cleavage was rejected because anisolewas found to undergo electrophilic substitution of the dibromoindole nucleus; a modified HF deprotection/cleavage procedure was developed and used instead. (C) Munksgaard 1993.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 01:32:52