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Titolo:
EFFECTS OF DEOXYGENATION ON ACTIVE AND PASSIVE CA2-CELL-ANEMIA RED-CELLS( TRANSPORT AND ON THE CYTOPLASMIC CA2+ LEVELS OF SICKLE)
Autore:
ETZION Z; TIFFERT T; BOOKCHIN RM; LEW VL;
Indirizzi:
YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT MED,1300 MORRIS PK AVE,ROOM 913U BRONX NY 10461 YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT MED,1300 MORRIS PK AVE,ROOM 913U BRONX NY 10461 UNIV CAMBRIDGE,PHYSIOL LAB CAMBRIDGE CB2 3EG ENGLAND
Titolo Testata:
The Journal of clinical investigation
fascicolo: 5, volume: 92, anno: 1993,
pagine: 2489 - 2498
SICI:
0021-9738(1993)92:5<2489:EODOAA>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
SIMULATED INTRACELLULAR CONDITIONS; CALCIUM-CHELATOR ESTERS; ADENOSINE 5'-TRIPHOSPHATE; HUMAN HEMOGLOBIN; CL COTRANSPORT; BLOOD-CELLS; MAGNESIUM; PUMP; POTASSIUM; RETICULOCYTES;
Keywords:
DEOXYGENATION; SICKLE CELL ANEMIA; RED CELLS; CYTOPLASMIC CA2+ BUFFERING; CA2+ PUMP; HEMOGLOBIN-S;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
56
Recensione:
Indirizzi per estratti:
Citazione:
Z. Etzion et al., "EFFECTS OF DEOXYGENATION ON ACTIVE AND PASSIVE CA2-CELL-ANEMIA RED-CELLS( TRANSPORT AND ON THE CYTOPLASMIC CA2+ LEVELS OF SICKLE)", The Journal of clinical investigation, 92(5), 1993, pp. 2489-2498

Abstract

Elevated [Ca2+], in deoxygenated sickle cell anemia (SS) red cells (RBCs) could trigger a major dehydration pathway via the Ca2+-sensitive K+ channel. But apart from an increase in calcium permeability, the effects of deoxygenation on the Ca2+ metabolism of sickle cells have notbeen previously documented. With the application of Ca-45(2+)-tracer flux methods and the combined use of the ionophore A23187, Co2+ ions, and intracellular incorporation of the Ca2+ chelator benz-2, in density-fractionated SS RBCs, we show here for the first time that upon deoxygenation, the mean [Ca2+]i level of SS discocytes was significantly increased, two- to threefold, from a normal range of 9.4 to 11.4 nM in the oxygenated cells, to a range of 21.8 to 31.7 nM in the deoxygenated cells, closer to K+ channel activatory levels. Unlike normal RBCs, deoxygenated SS RBCs showed a two- to fourfold increase in pump-leak Ca2+ turnover. Deoxygenation of the SS RBCs reduced their Ca2+ pump V(max), more so in reticulocyte- and discocyte-rich than in dense cell fractions, and decreased their cytoplasmic Ca2+ buffering. Analysis of these results suggests that both increased Ca2+ influx and reduced Ca2+ pump extrusion contribute to the [Ca2+]i elevation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 09:19:53